Anti-Cytokeratin 1/Krt1 Antibody Picoband™

Product Name

Anti-Cytokeratin 1/Krt1 Antibody Picoband™

View all KRT1 Antibodies

SKU/Catalog Number

A01639-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytokeratin 1/Krt1 Antibody Picoband™ catalog # A01639-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Cytokeratin 1/Krt1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01639-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Cytokeratin 1/Krt1 recombinant protein (Position: L223-Q439).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01639-1 is reactive to KRT1 in Mouse

Applications

A01639-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

66 kDa

Calculated molecular weight

66.039kDa

Background of KRT1

Keratin 1 (KRT1), also known as Cytokeratin 1, or Keratin, type II cytoskeletal 1, is a member of the keratin family. It is specifically expressed in the spinous and granular layers of the epidermis with family member keratin 10. Mutations in this gene have been associated with the variants ofbullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. And this type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Mouse
Immunofluorescence, 5 μg/ml, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody (A01639-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 1/Krt1 antigen affinity purified polyclonal antibody (Catalog # A01639-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 1/Krt1 at approximately 66 kDa. The expected band size for Cytokeratin 1/Krt1 is at 66 kDa.

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Figure 2. IHC analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody (A01639-1).
Cytokeratin 1/Krt1 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytokeratin 1/Krt1 Antibody (A01639-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IF analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody (A01639-1).
Cytokeratin 1/Krt1 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytokeratin 1/Krt1 Antibody (A01639-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For KRT1 (Source: Uniprot.org, NCBI)

Alternative Names

CK1; CK-1; cytokeratin 1; Cytokeratin-1; cytokeratin-1,67 kDa cytokeratin; EHK; EHK1; epidermolytic hyperkeratosis 1; EPPK; Hair alpha protein; K1; keratin 1; keratin, type II cytoskeletal 1; Keratin-1; KRT1A; KRTA; NEPPK; Type-II keratin Kb1 KRT1 CK1, EHK, EHK1, EPPK, K1A, NEPPK, KRT1 keratin 1 keratin, type II cytoskeletal 1|67 kDa cytokeratin|CK-1|cytokeratin 1|epidermolytic hyperkeratosis 1|hair alpha protein|keratin 1, type II|type-II keratin Kb1

For more info on KRT1, check out the KRT1 Infographic

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In this infographic, you will see the following information for KRT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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