Product Info Summary
SKU: | A02305-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 10 KRT10 Antibody
SKU/Catalog Number
A02305-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 10 KRT10 Antibody catalog # A02305-2. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 10 KRT10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02305-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Keratin 10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02305-2 is reactive to KRT10 in Human, Mouse, Rat
Applications
A02305-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
58.827kDa
Background of KRT10
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
XM Zhou, J. Biol. Chem., Oct 1988; 263: 15584 - 15589.
Felix B. M¨¹ller, Hum. Mol. Genet., Apr 2006; 15: 1133 - 1141.
J Reichelt, J. Cell Sci., Sep 1997; 110: 2175.
BP Korge, PNAS, Feb 1992; 89: 910.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of KRT10 using Anti-Cytokeratin 10 KRT10 Antibody (A02305-2).
ICC staining Cytokeratin 10 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Cytokeratin 10 KRT10 Antibody A02305-2
Western blot analysis of Cytokeratin 10 on Hela cell lysates using anti-Cytokeratin 10 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For KRT10 (Source: Uniprot.org, NCBI)
Gene Name
KRT10
Full Name
Keratin, type I cytoskeletal 10
Weight
58.827kDa
Superfamily
intermediate filament family
Alternative Names
BCIE; BIE; CK10; CK-10; cytokeratin 10; Cytokeratin-10; EHK; K10keratosis palmaris et plantaris; Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10; keratin, type I cytoskeletal 10; keratin-10; KPP KRT10 BCIE, BIE, CK10, EHK, K10, KPP keratin 10 keratin, type I cytoskeletal 10|CK-10|cytokeratin 10|keratin 10, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT10, check out the KRT10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 10 KRT10 Antibody (A02305-2)
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No publications found for A02305-2
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