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Product Info Summary
| SKU: | M04299 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 13 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04299
BM4581 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 13 Rabbit Monoclonal Antibody catalog # M04299. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 13 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04299)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GAC-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 13
Reactive Species
M04299 is reactive to KRT13 in Human, Mouse
Observed Molecular Weight
50 kDa
Calculated molecular weight
49.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04299 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: A431 cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Western blot analysis of Cytokeratin 13/KRT13 using anti-Cytokeratin 13/KRT13 antibody (M04299).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: mouse skin tissue lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 13/KRT13 antigen affinity purified monoclonal antibody (M04299) at 1:50000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cytokeratin 13/KRT13 at approximately 50 kDa. The expected band size for Cytokeratin 13/KRT13 is at 50 kDa.
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IHC analysis of KRT13 using anti-KRT13 antibody (M04299).
KRT13 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KRT13 Antibody (M04299) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Cytokeratin 13 Rabbit Monoclonal Antibody (M04299)
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5 Customer Q&As for Anti-Cytokeratin 13 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Cytokeratin 13 Rabbit Monoclonal antibody (M04299)?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We do provide the blocking peptide for product anti-Cytokeratin 13 Rabbit Monoclonal antibody (M04299). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-11
Question
I was wanting to use your anti-Cytokeratin 13 Rabbit Monoclonal antibody for WB for mouse tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse tongue identification?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
As indicated on the product datasheet, M04299 anti-Cytokeratin 13 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-10
Question
We are currently using anti-Cytokeratin 13 Rabbit Monoclonal antibody M04299 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The anti-Cytokeratin 13 Rabbit Monoclonal antibody (M04299) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-18
Question
Will M04299 anti-Cytokeratin 13 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Lewis
Verified customer
Asked: 2019-06-06
Answer
It shows on the product datasheet, M04299 anti-Cytokeratin 13 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-06
Question
I see that the anti-Cytokeratin 13 Rabbit Monoclonal antibody M04299 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-02-22
