Anti-Cytokeratin 14 (KRT14) (Squamous Cell Marker) Monoclonal Antibody

Product Name

Anti-Cytokeratin 14 (KRT14) (Squamous Cell Marker) Monoclonal Antibody

View all Cytokeratin 14 Antibodies

SKU/Catalog Number

M01432

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Cytokeratin 14 (KRT14) (Squamous Cell Marker) Monoclonal Antibody (Catalog # M01432). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Cytokeratin 14 (KRT14) (Squamous Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01432)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: KRT14/532

Isotype

IgG3, kappa

Immunogen

Recombinant full-length human KRT14 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01432 is reactive to KRT14 in Human, Mouse, Rat

Applications

M01432 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

51.561kDa

Background of Cytokeratin 14

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-Cytokeratin 14 Monoclonal Antibody (KRT14/532).

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Formalin-fixed, paraffin-embedded human Cervix stained with Anti-Cytokeratin 14 Monoclonal Antibody (KRT14/532).

Gene/Protein Information For KRT14 (Source: Uniprot.org, NCBI)

Alternative Names

CK14; CK-14; Cytokeratin 14; cytokeratin-14; EBS3; EBS4; K14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14; keratin, type I cytoskeletal 14; keratin-14; KRT14; NFJ KRT14 CK14, EBS3, EBS4, K14, NFJ keratin 14 keratin, type I cytoskeletal 14|cytokeratin 14|keratin 14, type I

For more info on KRT14, check out the KRT14 Infographic

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In this infographic, you will see the following information for KRT14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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