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- Table of Contents
KRT18 antibody (M01357-4) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of A431 cells using anti- Cytokeratin 18 antibody (M01357-4).
Overlay histogram showing A431 cells stained with M01357-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytokeratin 18 Antibody (M01357-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with M01357-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytokeratin 18 Antibody (M01357-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6)
KRT18 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
KRT18 antibody was positively detected by WB in:
human placenta tissue, human Caco-2 whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KRT18 antibody was positively detected by IHC in:
human rectal cancer tissue, human lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
KRT18 antibody was positively detected by ICC/IF in:
Hela cell
KRT18 antibody was positively detected by IF in:
human placenta tissue
KRT18 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6)
View all KRT18 Antibodies
View all KRT18 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
7I6
Isotype
Mouse IgG1
Immunogen
E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430). Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
48 kDa
Calculated Molecular Weight
48.1 kDa
Background of KRT18
Keratin 18, mapped to 12q13.13, is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Cite This Product
Anti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01357-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
91%
High Homology
Cow
91%
High Homology
Sheep
91%
High Homology
Dog
90%
High Homology
Mouse
88%
Moderate Homology
Zebrafish
54%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for KRT18 antibody
Immunohistochemistry (IHC) protocol for KRT18 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for KRT18 antibody
Immunofluorescence (IF) protocol for KRT18 antibody
Flow Cytometry (FC) protocol for KRT18 antibody
Publications
Application:
Species:
Pretreatment with Inflammatory Factors Altered the Secretome of Human Amniotic Epithelial Cells
Published: 2024-06-19
Journal: Tissue Engineering Part C-Methods
Application: IF
Impact Factor: 2.70
Species: Human
PMID: 38756098
Human amniotic epithelial cells improve fertility in an intrauterine adhesion mouse model
Published: 2019-08-14
Journal: Stem Cell Research & Therapy
Application: IF
Impact Factor: 4.63
Species: Human
PMID: 31412924
Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses
Published: 2018-03-01
Journal: PLoS One
Application: IF
Impact Factor: 2.77
Species: Goose
PMID: 29494688
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Product Promise
We promise Anti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6) performs excellently in relevant applications for Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M01357-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026