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Product Info Summary
| SKU: | M02828 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 20 KRT20 Rabbit Monoclonal Antibody
View all Cytokeratin 20 Antibodies
SKU/Catalog Number
M02828
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 20 KRT20 Rabbit Monoclonal Antibody catalog # M02828. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 20 KRT20 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02828)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CDI-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 20
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02828 is reactive to KRT20 in Human, Rat
Applications
M02828 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
30 kDa
Calculated molecular weight
48.487kDa
Background of Cytokeratin 20
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of Hela cells, using Cytokeratin 20 Antibody .
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Western blot analysis of Cytokeratin 20 expression in SW480 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human colon, using Cytokeratin 20 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For KRT20 (Source: Uniprot.org, NCBI)
Gene Name
KRT20
Full Name
Keratin, type I cytoskeletal 20
Weight
48.487kDa
Superfamily
intermediate filament family
Alternative Names
CD20; CK20; CK-20; cytokeratin-20; K20cytokeratin 20; keratin 20; keratin, type I cytoskeletal 20; keratin-20; KRT21; MGC35423; Protein IT KRT20 CD20, CK-20, CK20, K20, KRT21 keratin 20 keratin, type I cytoskeletal 20|cytokeratin 20|keratin 20, type I|protein IT
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT20, check out the KRT20 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 20 KRT20 Rabbit Monoclonal Antibody (M02828)
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No publications found for M02828
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Cytokeratin 20 KRT20 Rabbit Monoclonal Antibody
Question
Can M02828 be used in the IHC-P technique?
Verified customer
Asked: 2022-07-12
Answer
Yes,tThe Anti-Cytokeratin 20 KRT20 Rabbit Monoclonal Antibody (M02828) can be used for IHC-P. Please find the result image on the product page. https://www.bosterbio.com/anti-cytokeratin-20-rabbit-monoclonal-antibody-m02828-boster.html
Boster Scientific Support
Answered: 2022-07-13
Question
See below the WB image, lot number and protocol we used for intestine using anti-Cytokeratin 20 Rabbit Monoclonal antibody M02828. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-07
Question
Will M02828 anti-Cytokeratin 20 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Anderson
Verified customer
Asked: 2019-10-01
Answer
You can see on the product datasheet, M02828 anti-Cytokeratin 20 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-01
Question
Will anti-Cytokeratin 20 Rabbit Monoclonal antibody M02828 work for IF with intestine?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
According to the expression profile of intestine, KRT20 is highly expressed in intestine. So, it is likely that anti-Cytokeratin 20 Rabbit Monoclonal antibody M02828 will work for IF with intestine.
Boster Scientific Support
Answered: 2019-02-19
Question
My question regarding product M02828, anti-Cytokeratin 20 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Williams
Verified customer
Asked: 2018-08-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02828 anti-Cytokeratin 20 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for intestine using anti-Cytokeratin 20 Rabbit Monoclonal antibody M02828. Let me know if you need anything else.
P. Mangal
Verified customer
Asked: 2013-12-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-20
