Product Info Summary
| SKU: | A05711 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Vam6/Vps39-like protein VPS39 Antibody
SKU/Catalog Number
A05711
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Vam6/Vps39-like protein VPS39 Antibody (Catalog # A05711). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VPS39 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Vam6/Vps39-like protein VPS39 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05711)
Host
Rabbit
Contents
VPS39 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VPS39 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human VPS39. The immunogen is located within amino acids 710 - 760 of VPS39.
Cross-reactivity
At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1.
Reactive Species
A05711 is reactive to VPS39 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
101.8 kDa
Background of VPS39
VPS39 is the mammalian homolog of the yeast vacuolar protein VAM6, that in mammals, promotes lysosome clustering and fusion in vivo. VPS39 is also thought to modulate the TGF-beta response by coupling the TGF-beta receptor complex to the Smad pathway. Similar to the related protein TRAP1, the VPS39 protein is essential for early embryonic development, with mice lacking VPS39 dying at or before E6.5.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05711 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VPS39 antibody can be used for detection of VPS39 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of VPS39 in rat liver tissue lysate with VPS39 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Click image to see more details
Immunohistochemistry of VPS39 in mouse liver tissue with VPS39 antibody at 5 μg/mL.
Click image to see more details
Immunofluorescence of VPS39 in mouse liver tissue with VPS39 antibody at 20 μg/mL.
Click image to see more details
Immunofluorescence of VPS39 in human liver tissue with VPS39 antibody at 5 μg/ml.
Green: VPS39 antibody (A05711)
Red: Phylloidin staining
Blue: DAPI staining
Specific Publications For Anti-Vam6/Vps39-like protein VPS39 Antibody (A05711)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Vam6/Vps39-like protein VPS39 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Vam6/Vps39-like protein VPS39 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question

