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Product Info Summary
| SKU: | M00398 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody
SKU/Catalog Number
M00398
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody (Catalog # M00398). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Pig, Mouse, Rat, Hamster, Cow, Dog, Sheep, Ferret.
Storage & Handling
Store at 2 to 8°C. Antibody is stable for 24 months.
Cite This Product
Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00398)
Host
Mouse
Contents
Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format.
Clonality
Monoclonal
Clone Number
Clone: C-50
Isotype
IgG1, kappa
Immunogen
Cytoskeletal preparation from HeLa cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00398 is reactive to in Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep
Applications
M00398 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
62378 MW
Background of
This monoclonal antibody reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Reconstitute with 1ml water (15 minutes at room temperature).
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Anti-Cytokeratin 5/8 Monoclonal Antibody (C-50).
Specific Publications For Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody (M00398)
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No publications found for M00398
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody
Question
We are currently using anti-Cytokeratin 5/8 Monoclonal antibody M00398 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says bovine, canine, hamster, human, mouse, pig, rat. Is it true that the antibody can work on zebrafish tissues as well?
N. Brown
Verified customer
Asked: 2020-04-07
Answer
The anti-Cytokeratin 5/8 Monoclonal antibody (M00398) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
We have observed staining in rat ectocervix. Do you have any suggestions? Is anti-Cytokeratin 5/8 Monoclonal antibody supposed to stain ectocervix positively?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
From what I have seen in literature ectocervix does express KRT5. From what I have seen in Uniprot.org, KRT5 is expressed in ectocervix, brain pancreas, lymphoblast, liver, among other tissues. Regarding which tissues have KRT5 expression, here are a few articles citing expression in various tissues:
Brain, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Boster Scientific Support
Answered: 2020-01-02
