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Product Info Summary
| SKU: | A00998-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-D4DR DRD4 Antibody
SKU/Catalog Number
A00998-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-D4DR DRD4 Antibody catalog # A00998-1. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-D4DR DRD4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00998-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DRD4. AA range:355-404
Reactive Species
A00998-1 is reactive to DRD4 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00998-1 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Immunofluorescence analysis of MCF7 cells, using DRD4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using D4DR Polyclonal Antibody
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Western Blot analysis of K562 cells using D4DR Polyclonal Antibody
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Western blot analysis of lysates from MCF-7 and HUVEC cells, using DRD4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from RAW264.7cells using DRD4 antibody.
Specific Publications For Anti-D4DR DRD4 Antibody (A00998-1)
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Customer Reviews
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3 Customer Q&As for Anti-D4DR DRD4 Antibody
Question
We are currently using anti-D4DR antibody A00998-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
The anti-D4DR antibody (A00998-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-23
Question
Would anti-D4DR antibody A00998-1 work on zebrafish WB with cerebellum?
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
Our lab technicians have not tested anti-D4DR antibody A00998-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-D4DR antibody A00998-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cerebellum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-14
Question
Is there a BSA free version of anti-D4DR antibody A00998-1 available?
K. Singh
Verified customer
Asked: 2017-07-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-D4DR antibody A00998-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-24
