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Product Info Summary
| SKU: | A00910 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-DAF CD55 Antibody
SKU/Catalog Number
A00910
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DAF CD55 Antibody (Catalog # A00910). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
DAF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-DAF CD55 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00910)
Host
Rabbit
Contents
DAF antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF. The immunogen is located within amino acids 360 - 410 of DAF.
Cross-reactivity
DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.
Reactive Species
A00910 is reactive to CD55 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
41.4 kDa
Background of CD55
The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00910 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.
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Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.
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Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.
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Western blot analysis of DAF1 expression in HeLa cell lysate with DAF1 antibody at (A) 1 and (B) 2 μg/ml.
Specific Publications For Anti-DAF CD55 Antibody (A00910)
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