Anti-DAO Antibody Picoband™

Product Name

Anti-DAO Antibody Picoband™

View all DAO Antibodies

SKU/Catalog Number

A00773-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DAO Antibody Picoband™ catalog # A00773-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-DAO Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00773-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human DAO recombinant protein (Position: H20-L347).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00773-4 is reactive to DAO in Human, Rat

Applications

A00773-4 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

39.474kDa

Background of DAO

D-amino acid oxidase (DAAO; also OXDA, DAMOX) is an enzyme with the function on a molecular level to oxidize D-amino acids to the corresponding α-keto acids, producing ammonia and hydrogen peroxide. This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of DAO using anti-DAO antibody (A00773-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAO antigen affinity purified polyclonal antibody (Catalog # A00773-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAO at approximately 36 kDa. The expected band size for DAO is at 39 kDa.

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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-DAO antibody (A00773-4).
Overlay histogram showing HL-60 cells stained with A00773-4 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAO Antibody (A00773-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For DAO (Source: Uniprot.org, NCBI)

Alternative Names

DAAO; D-Amino Acid Oxidase; D-amino-acid oxidase; DAMOX; DAMOXOXDA; DAO; EC 1.4.3; EC 1.4.3.3; MGC35381; OXDA DAO DAAO, DAMOX, OXDA D-amino acid oxidase D-amino-acid oxidase

For more info on DAO, check out the DAO Infographic

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In this infographic, you will see the following information for DAO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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