Anti-Dapper homolog 3 Dact3 Antibody

Product Name

Anti-Dapper homolog 3 Dact3 Antibody

View all DACT3 Antibodies

SKU/Catalog Number

A11557

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Dapper homolog 3 Dact3 Antibody (Catalog # A11557). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Dact3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Dapper homolog 3 Dact3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11557)

Host

Rabbit

Contents

Dact3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Dact3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Dact3. The immunogen is located within amino acids 370 - 420 of Dact3.

Reactive Species

A11557 is reactive to DACT3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

64.9 kDa

Background of DACT3

The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact3, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact3 is expressed in the ventral region of maturing somites, limb bud and branchial arch mesenchyme, embryonic CNS, and the adult brain. Recent evidence shows that Dact3 acts as a negative regulator Wnt/beta-catenin signaling that is repressed at the transcriptional level in colorectal cancer and this repression is associated with bivalent histone modifications. This repression can be reversed by pharmacological agents that targets both histone methylation and deacetylation, suggesting that Dact3 may be a potential target for therapeutic treatment of this cancer. At least three isoforms of Dact3 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11557 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Dact3 antibody can be used for detection of Dact3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information DACT3

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