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Product Info Summary
| SKU: | M02069 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DAZL Monoclonal Antibody
SKU/Catalog Number
M02069
BM5226 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DAZL Monoclonal Antibody catalog # M02069. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DAZL Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02069)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACBF-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DAZL RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
Reactive Species
M02069 is reactive to DAZL in Human
Observed Molecular Weight
33 kDa
Calculated molecular weight
33.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02069 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human testis
Validation Images & Assay Conditions
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Western blot analysis of DAZL expression in human testis lysate.
Specific Publications For Anti-DAZL Monoclonal Antibody (M02069)
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5 Customer Q&As for Anti-DAZL Monoclonal Antibody
Question
We are currently using anti-DAZL Monoclonal antibody M02069 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-DAZL Monoclonal antibody (M02069) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
My question regarding product M02069, anti-DAZL Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02069 anti-DAZL Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-10
Question
What sample was tested in WB and IHC applications with M02069?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of DAZL expression in human testis lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Is a blocking peptide available for product anti-DAZL Monoclonal antibody (M02069)?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
We do provide the blocking peptide for product anti-DAZL Monoclonal antibody (M02069). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-08
Question
Here is the WB image, lot number and protocol we used for testis using anti-DAZL Monoclonal antibody M02069. Please let me know if you require anything else.
J. Brown
Verified customer
Asked: 2016-05-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-13
