Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband™

ACBP antibody

Boster Bio Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband™ catalog # A01267. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A01267
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband™

View all ACBP Antibodies

SKU/Catalog Number

A01267

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband™ catalog # A01267. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01267)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human DBI recombinant protein (Position: S2-I87). Human DBI shares 77.9% amino acid (aa) sequence identity with both mouse and rat DBI.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A01267 is reactive to DBI in Human

Applications

A01267 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

10 kDa

Calculated molecular weight

10.044kDa

Background of ACBP

Acyl-CoA-binding protein is a protein that in humans is encoded by the DBI gene. This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For DBI (Source: Uniprot.org, NCBI)

Gene Name

DBI

Full Name

Acyl-CoA-binding protein

Weight

10.044kDa

Superfamily

ACBP family

Alternative Names

acyl-Coenzyme A binding domain containing 1; acyl-Coenzyme A bindingprotein); CCK-RP; cholecystokinin-releasing peptide, trypsin-sensitive; diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein); Diazepam-binding inhibitor; endozepine; EP; GABA receptor modulator; MGC70414 DBI ACBD1, ACBP, CCK-RP, EP diazepam binding inhibitor, acyl-CoA binding protein acyl-CoA-binding protein|GABA receptor modulator|acyl coenzyme A binding protein|acyl-Coenzyme A binding domain containing 1|cholecystokinin-releasing peptide, trypsin-sensitive|diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein)|diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein)|diazepam-binding inhibitor|endozepine|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DBI, check out the DBI Infographic

DBI infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DBI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband™

Question

We are currently using anti-Diazepam Binding Inhibitor/DBI antibody A01267 for human tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

The anti-Diazepam Binding Inhibitor/DBI antibody (A01267) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-13

Order DetailsPrice
A01267

100μg

$370
A01267-10ug

10μg sample (liquid)

$99
A01267-Biotin

100 μg Biotin conjugated

$570
A01267-Cy3

100 μg Cy3 conjugated

$570
A01267-Dylight488

100 μg Dylight488 conjugated

$570
A01267-Dylight550

100 μg Dylight550 conjugated

$570
A01267-Dylight594

100 μg Dylight594 conjugated

$570
A01267-FITC

100 μg FITC conjugated

$570
A01267-HRP

100 μg HRP conjugated

$570
A01267-APC

100 μg APC conjugated

$670
A01267-PE

100 μg PE conjugated

$670
A01267-carrier-free

Carrier Free

$370

More conjugates/formats

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A01267
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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