Anti-DCAF7 Rabbit Monoclonal Antibody

Product Name

Anti-DCAF7 Rabbit Monoclonal Antibody

View all WDR68 Antibodies

SKU/Catalog Number

M08577

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-DCAF7 Rabbit Monoclonal Antibody catalog # M08577. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DCAF7 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08577)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

27D63

Isotype

IgG

Immunogen

A synthesized peptide derived from human DCAF7

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08577 is reactive to DCAF7 in Human, Mouse, Rat

Applications

M08577 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

38.926kDa

Background of WDR68

Putative transcription factor involved in pancreas development and function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of DCAF7 using anti-DCAF7 antibody (M08577).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF7 antigen affinity purified monoclonal antibody (Catalog # M08577) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCAF7 at approximately 39 kDa. The expected band size for DCAF7 is at 39 kDa.

Gene/Protein Information For DCAF7 (Source: Uniprot.org, NCBI)

Alternative Names

DDB1 and CUL4 associated factor 7; DDB1- and CUL4-associated factor 7; HAN11AN11; human anthocyanin; seven-WD-repeat protein of the AN11 family-1; SWAN-1; WD repeat domain 68; WD repeat-containing protein 68; WD repeat-containing protein An11 homolog; WDR68; WD-repeat protein DCAF7 AN11, HAN11, SWAN-1, WDR68 DDB1 and CUL4 associated factor 7 DDB1- and CUL4-associated factor 7|WD repeat-containing protein 68|WD repeat-containing protein An11 homolog|human anthocyanin|seven-WD-repeat protein of the AN11 family-1

For more info on DCAF7, check out the DCAF7 Infographic

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In this infographic, you will see the following information for DCAF7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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