Product Info Summary
SKU: | A09974 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-DCI ECI1 Antibody
SKU/Catalog Number
A09974
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DCI ECI1 Antibody catalog # A09974. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DCI ECI1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09974)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human DCI.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09974 is reactive to ECI1 in Human
Applications
A09974 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
32.816kDa
Background of DCI
Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Yazaki M., Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
Ishikawa K., DNA Res. 4:307-313(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of ECI1 using Anti-DCI ECI1 Antibody (A09974).
Immunohistochemistry (IHC) analysis of paraffin-embedded human lung cancerClick image to see more details
Figure 3. Immunohistochemistry validation of ECI1 using Anti-DCI ECI1 Antibody (A09974).
Immunohistochemistry (IHC) analysis of paraffin-embedded human lung cancerClick image to see more details
Figure 4. Immunohistochemistry validation of ECI1 using Anti-DCI ECI1 Antibody (A09974).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human LiverClick image to see more details
Figure 1. Western blotting validation for Anti-DCI ECI1 Antibody A09974
Western Blot (WB) analysis of HepG2 cells using DCI polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ECI1 (Source: Uniprot.org, NCBI)
Gene Name
ECI1
Full Name
Enoyl-CoA delta isomerase 1, mitochondrial
Weight
32.816kDa
Superfamily
enoyl-CoA hydratase/isomerase family
Alternative Names
acetylene-allene isomerase; D2-enoyl-CoA isomerase; D3; DCI3,2-trans-enoyl-CoA isomerase3,2 trans-enoyl-CoA isomerase; Delta(2)-enoyl-CoA isomerase; Delta(3); delta3, delta2-enoyl-CoA isomerase; dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase); dodecenoyl-CoA isomerase3,2-trans-enoyl-CoA isomerase, mitochondrial; dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase); EC 5.3.3.8; enoyl-CoA delta isomerase 13,2 trans-enoyl-Coenzyme A isomerase ECI1 DCI enoyl-CoA delta isomerase 1 enoyl-CoA delta isomerase 1, mitochondrial|3,2 trans-enoyl-Coenzyme A isomerase|3,2-trans-enoyl-CoA isomerase, mitochondrial|D3,D2-enoyl-CoA isomerase|acetylene-allene isomerase|delta(3),Delta(2)-enoyl-CoA isomerase|dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase)|dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ECI1, check out the ECI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ECI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DCI ECI1 Antibody (A09974)
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No publications found for A09974
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