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Product Info Summary
|Reactive Species:||Human, Mouse|
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Anti-MC2 receptor/MC2R Antibody Picoband™
Boster Bio Anti-MC2 receptor/MC2R Antibody Picoband™ catalog # A02306-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MC2 receptor/MC2R Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02306-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human MC2 receptor (268-297aa NAVIDPFIYAFRSPELRDAFKKMIFCSRYW), different from the related mouse sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A02306-1 is reactive to MC2R in Human, Mouse
A02306-1 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MC2 receptor using anti-MC2 receptor antibody (A02306-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC2 receptor antigen affinity purified polyclonal antibody (Catalog # A02306-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC2 receptor at approximately 39KD. The expected band size for MC2 receptor is at 34KD.
Gene/Protein Information For MC2R (Source: Uniprot.Org, NCBI)
Adrenocorticotropic hormone receptor
G-protein coupled receptor 1 family
ACTH receptor; ACTHR; ACTH-R; ACTHRcorticotropin receptor; adrenocorticotropic hormone receptor; Adrenocorticotropin receptor; MC2 receptor; MC2R; MC2-R; melanocortin 2 receptor (adrenocorticotropic hormone); Melanocortin receptor 2; Melanocortin-2 R; Melanocortin2R; Melanocortin-2R; MGC125798 MC2R ACTHR melanocortin 2 receptor adrenocorticotropic hormone receptor|ACTH receptor|MC2 receptor|adrenocorticotropin receptor|corticotropin receptor|melanocortin 2 receptor (adrenocorticotropic hormone)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MC2R, check out the MC2R Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MC2R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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