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Product Info Summary
| SKU: | M03136 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DDAH1 Monoclonal Antibody
SKU/Catalog Number
M03136
BM5493 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DDAH1 Monoclonal Antibody catalog # M03136. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDAH1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03136)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEAG-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DDAH1 Hydrolyzes N (G),N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Reactive Species
M03136 is reactive to DDAH1 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
37 kDa
Calculated molecular weight
31.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03136 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:40
FC 1:30
Positive Control
WB: human 293T whole cell, human Hela whole cell, monkey COS-7 whole cell, human SH-SY5Y whole cell
Validation Images & Assay Conditions
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Western blot analysis of DDAH1 using anti-DDAH1 antibody (M03136).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH1 antigen affinity purified monoclonal antibody (Catalog # M03136) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDAH1 at approximately 37 kDa. The expected band size for DDAH1 is at 31 kDa.
Specific Publications For Anti-DDAH1 Monoclonal Antibody (M03136)
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4 Customer Q&As for Anti-DDAH1 Monoclonal Antibody
Question
Would M03136 anti-DDAH1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
As indicated on the product datasheet, M03136 anti-DDAH1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-15
Question
Is there a BSA free version of anti-DDAH1 Monoclonal antibody M03136 available?
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DDAH1 Monoclonal antibody M03136 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-02
Question
I see that the anti-DDAH1 Monoclonal antibody M03136 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-09-11
Question
Will anti-DDAH1 Monoclonal antibody M03136 work for WB with endometrial cancer?
C. Anderson
Verified customer
Asked: 2015-12-24
Answer
According to the expression profile of endometrial cancer, DDAH1 is highly expressed in endometrial cancer. So, it is likely that anti-DDAH1 Monoclonal antibody M03136 will work for WB with endometrial cancer.
Boster Scientific Support
Answered: 2015-12-24
