Anti-DDAH2 Monoclonal Antibody

DDAH2 antibody

Boster Bio Anti-DDAH2 Monoclonal Antibody catalog # M07566. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M07566
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, WB

Product Name

Anti-DDAH2 Monoclonal Antibody

View all DDAH2 Antibodies

SKU/Catalog Number

M07566

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DDAH2 Monoclonal Antibody catalog # M07566. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDAH2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07566)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEGG-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human DDAH2 Hydrolyzes N (G),N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M07566 is reactive to DDAH2 in Human, Mouse, Rat

Applications

M07566 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

29.644kDa

Background of DDAH2

Putative transcription factor involved in pancreas development and function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For DDAH2 (Source: Uniprot.org, NCBI)

Gene Name

DDAH2

Full Name

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Weight

29.644kDa

Superfamily

DDAH family

Alternative Names

DDAHII; dimethylargininase-2; dimethylarginine dimethylaminohydrolase 2G6a; dimethylarginine dimethylaminohydrolase II; EC 3.5.3.18; G6a; N(G); N(G)-dimethylarginine dimethylaminohydrolase 2; NG30; NG-dimethylarginine dimethylamino hydrolase homolog; S-phase protein DDAH2 DDAH, DDAHII, G6a, HEL-S-277, NG30 dimethylarginine dimethylaminohydrolase 2 N(G),N(G)-dimethylarginine dimethylaminohydrolase 2|S-phase protein|dimethylargininase-2|dimethylarginine dimethylaminohydrolase II|epididymis secretory protein Li 277|testis tissue sperm-binding protein Li 54e

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DDAH2, check out the DDAH2 Infographic

DDAH2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DDAH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-DDAH2 Monoclonal Antibody

Question

We want to test anti-DDAH2 Monoclonal antibody M07566 on rat umbilical cord blood for research purposes, then I may be interested in using anti-DDAH2 Monoclonal antibody M07566 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

The products we sell, including anti-DDAH2 Monoclonal antibody M07566, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-22

Question

I was wanting to use your anti-DDAH2 Monoclonal antibody for IP for rat umbilical cord blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat umbilical cord blood identification?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

It shows on the product datasheet, M07566 anti-DDAH2 Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat umbilical cord blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-10

Question

Will anti-DDAH2 Monoclonal antibody M07566 work for IP with umbilical cord blood?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

According to the expression profile of umbilical cord blood, DDAH2 is highly expressed in umbilical cord blood. So, it is likely that anti-DDAH2 Monoclonal antibody M07566 will work for IP with umbilical cord blood.

Boster Scientific Support

Answered: 2019-09-24

Question

Is a blocking peptide available for product anti-DDAH2 Monoclonal antibody (M07566)?

R. Jha

Verified customer

Asked: 2015-04-13

Answer

We do provide the blocking peptide for product anti-DDAH2 Monoclonal antibody (M07566). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-04-13

Question

Would anti-DDAH2 Monoclonal antibody M07566 work on bovine IP with liver?

S. Parker

Verified customer

Asked: 2014-10-10

Answer

Our lab technicians have not validated anti-DDAH2 Monoclonal antibody M07566 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-DDAH2 Monoclonal antibody M07566 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-10-10

Question

See attached the WB image, lot number and protocol we used for umbilical cord blood using anti-DDAH2 Monoclonal antibody M07566. Please let me know if you require anything else.

T. Wu

Verified customer

Asked: 2013-04-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-04-01

Order DetailsPrice
M07566

100μL

$370
M07566-30ul

30μL sample

$99
M07566-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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