Product Info Summary
SKU: | A04605-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-DDX21 Antibody Picoband™
SKU/Catalog Number
A04605-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DDX21 Antibody Picoband™ catalog # A04605-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DDX21 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04605-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DDX21 recombinant protein (Position: E395-Q660).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04605-2 is reactive to DDX21 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
86 kDa
Calculated molecular weight
Background of DDX21
Nucleolar RNA helicase 2 is an enzyme that in humans is encoded by the DDX21 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04605-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Positive Control
Validation Images & Assay Conditions
![a04605 2 ddx21 primary antibodies wb testing 1 a04605 2 ddx21 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a04605-2-ddx21-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of DDX21 using anti-DDX21 antibody (A04605-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX21 antigen affinity purified polyclonal antibody (Catalog # A04605-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX21 at approximately 86 kDa. The expected band size for DDX21 is at 86 kDa.
![a04605 2 ddx21 primary antibodies if testing 2 a04605 2 ddx21 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a04605-2-ddx21-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of DDX21 using anti-DDX21 antibody (A04605-2).
DDX21 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX21 Antibody (A04605-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a04605 2 ddx21 primary antibodies fcm testing 3 a04605 2 ddx21 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a04605-2-ddx21-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of A431 cells using anti-DDX21 antibody (A04605-2).
Overlay histogram showing A431 cells stained with A04605-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX21 Antibody (A04605-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DDX21 (Source: Uniprot.org, NCBI)
Gene Name
DDX21
Full Name
Nucleolar RNA helicase 2
Weight
Superfamily
DEAD box helicase family
Alternative Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD box protein 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21; DKFZp686F21172; EC 3.6.1; EC 3.6.4.13; Gu protein; GUA; gu-alpha; GURDB; nucleolar RNA helicase 2; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu; RH-II/GU; RH-II/GuA; RNA helicase II/Gu alpha DDX21 GUA, GURDB, RH-II/GU, RH-II/GuA DExD-box helicase 21 nucleolar RNA helicase 2|DEAD (Asp-Glu-Ala-Asp) box helicase 21|DEAD (Asp-Glu-Ala-Asp) box polypeptide 21|DEAD box protein 21|DEAD-box helicase 21|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21|Gu protein|RH II/Gu|RNA helicase II/Gu alpha|gu-alpha|nucleolar RNA helicase Gu|nucleolar RNA helicase II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX21, check out the DDX21 Infographic
![DDX21 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX21 Antibody Picoband™ (A04605-2)
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