Product Info Summary
SKU: | A11206 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-DDX51 Antibody
SKU/Catalog Number
A11206
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-DDX51 Antibody (Catalog # A11206). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX51 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11206)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human DDX51.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11206 is reactive to Ddx51 in Human
Applications
A11206 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
70.368kDa
Background of Ddx51
ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits By similarity.
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells, using DDX51 antibody A11206.
Protein Target Info & Infographic
Gene/Protein Information For Ddx51 (Source: Uniprot.org, NCBI)
Gene Name
Ddx51
Full Name
ATP-dependent RNA helicase DDX51
Weight
70.368kDa
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase DDX51; DEAD (Asp-Glu-Ala-Asp) box polypeptide 51; DEAD box protein 51; DKFZp686N2081; EC 3.6.1; EC 3.6.4.13; MGC42193
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ddx51, check out the Ddx51 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ddx51: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX51 Antibody (A11206)
Hello CJ!
No publications found for A11206
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-DDX51 Antibody
Question
My question regarding product A11206, anti-DDX51 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11206 anti-DDX51 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-05
Question
I was wanting to use your anti-DDX51 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
C. Wu
Verified customer
Asked: 2019-09-09
Answer
As indicated on the product datasheet, A11206 anti-DDX51 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-09
Question
We are currently using anti-DDX51 antibody A11206 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
The anti-DDX51 antibody (A11206) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-06
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-DDX51 antibody A11206. Let me know if you need anything else.
L. Moore
Verified customer
Asked: 2018-03-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-13