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Product Info Summary
| SKU: | A09938 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-DDX60 Antibody
SKU/Catalog Number
A09938
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DDX60 Antibody (Catalog # A09938). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DDX60 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DDX60 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09938)
Host
Rabbit
Contents
DDX60 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60. The immunogen is located within amino acids 1140 - 1190 of DDX60.
Cross-reactivity
At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms.
Reactive Species
A09938 is reactive to DDX60 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
197.9 kDa
Background of DDX60
DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09938 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DDX60 in A20 cell lysate with DDX60 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of DDX60 in A20 cells with DDX60 antibody at 20 μg/mL.
Specific Publications For Anti-DDX60 Antibody (A09938)
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