Product Info Summary
SKU: | A02731-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Dectin-1/CLEC7A Antibody Picoband™
View all Dectin-1/CLEC7A Antibodies
SKU/Catalog Number
A02731-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Dectin-1/CLEC7A Antibody Picoband™ catalog # A02731-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Dectin-1/CLEC7A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02731-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Dectin-1/CLEC7A recombinant protein (Position: M1-C233).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02731-2 is reactive to Clec7a in Human
Applications
A02731-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
27.42kDa
Background of Dectin-1/CLEC7A
C-type lectin domain family 7 member A or Dectin-1 is a protein that in humans is encoded by the CLEC7A gene. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Dectin-1/CLEC7A using anti-Dectin-1/CLEC7A antibody (A02731-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dectin-1/CLEC7A antigen affinity purified polyclonal antibody (Catalog # A02731-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dectin-1/CLEC7A at approximately 36 kDa. The expected band size for Dectin-1/CLEC7A is at 28 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of U937 cells using anti-Dectin-1/CLEC7A antibody (A02731-2).
Overlay histogram showing U937 cells stained with A02731-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dectin-1/CLEC7A Antibody (A02731-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For Clec7a (Source: Uniprot.org, NCBI)
Gene Name
Clec7a
Full Name
C-type lectin domain family 7 member A
Weight
27.42kDa
Alternative Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1 Clec7a|BG, BGR, Clecsf, Clecsf12, beta-, beta-GR, dectin|C-type lectin domain family 7, member a|C-type lectin domain family 7 member A|C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12|C-type lectin superfamily member 12|DC-associated C-type lectin 1|beta-glucan receptor|dectin-1|dendritic cell-associated C-type lectin 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Clec7a, check out the Clec7a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Clec7a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dectin-1/CLEC7A Antibody Picoband™ (A02731-2)
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