Anti-DEDAF RYBP Antibody

Product Name

Anti-DEDAF RYBP Antibody

View all RYBP/DEDAF Antibodies

SKU/Catalog Number

A04316

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DEDAF RYBP Antibody (Catalog # A04316). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

DEDAF antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DEDAF RYBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04316)

Host

Rabbit

Contents

DEDAF Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DEDAF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DEDAF. The immunogen is located within the last 50 amino acids of DEDAF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ERAP2 antibody is human reactive. Multiple isoforms of ERAP2 are known to exist. ERAP2 antibody is predicted to not cross-react with ERAP1.

Reactive Species

A04316 is reactive to RYBP in Human, Mouse, Rat

Applications

A04316 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

24.822kDa

Background of RYBP/DEDAF

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DEDAF can be used for detection of DEDAF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of DEDAF expression in human A549 (lane A)

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Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 μg/mL.

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Immunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 μg/mL.

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Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 10 μg/mL.

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Immunofluorescence of DEDAF in mouse liver tissue with DEDAF antibody at 20 μg/ml.

Gene/Protein Information For RYBP (Source: Uniprot.org, NCBI)

Alternative Names

AAP1; APAP-1; Apoptin-associating protein 1; Death effector domain-associated factor; DEDAFYY1 and E4TF1-associated factor 1; RING1 and YY1 binding protein; RING1 and YY1-binding protein; ring1 interactor RYBP; YEAF1DED-associated factor; YY1 and E4TF1 associated factor 1 RYBP AAP1, APAP-1, DEDAF, YEAF1 RING1 and YY1 binding protein RING1 and YY1-binding protein|DED-associated factor|YY1 and E4TF1 associated factor 1|apoptin-associating protein 1|death effector domain-associated factor|ring1 interactor RYBP

For more info on RYBP, check out the RYBP Infographic

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In this infographic, you will see the following information for RYBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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