Click image to see more details
Product Info Summary
| SKU: | A07422 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Dematin DMTN Antibody
SKU/Catalog Number
A07422
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Dematin DMTN Antibody catalog # A07422. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dematin DMTN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07422)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Dematin. AA range:356-405
Reactive Species
A07422 is reactive to DMTN in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
45.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07422 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HUVEC cells, using Dematin Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using Dematin Polyclonal Antibody
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Western blot analysis of lysates from 293 cells, using Dematin Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Dematin Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Dematin DMTN Antibody (A07422)
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7 Customer Q&As for Anti-Dematin DMTN Antibody
Question
I have a question about product A07422, anti-Dematin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07422 anti-Dematin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Dematin antibody A07422. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-02
Question
Is this A07422 anti-Dematin antibody reactive to the isotypes of DMTN?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The immunogen of A07422 anti-Dematin antibody is Synthesized peptide derived from human Dematin around the non-phosphorylation site of S403. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-03
Question
Will anti-Dematin antibody A07422 work on goat WB with platelet?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
Our lab technicians have not validated anti-Dematin antibody A07422 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Dematin antibody A07422 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat platelet in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-01
Question
Is there a BSA free version of anti-Dematin antibody A07422 available?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Dematin antibody A07422 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-29
Question
Does A07422 anti-Dematin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Huang
Verified customer
Asked: 2016-03-25
Answer
You can see on the product datasheet, A07422 anti-Dematin antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-25
Question
I see that the anti-Dematin antibody A07422 works with WB, what is the protocol used to produce the result images on the product page?
S. Miller
Verified customer
Asked: 2015-09-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-09-24
