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Product Info Summary
| SKU: | A08594 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-DEPDC1 Antibody (N-term)
SKU/Catalog Number
A08594
Size
80 µl
Description
Boster Bio Anti-DEPDC1 Antibody (N-term) (Catalog # A08594). Tested in IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DEPDC1 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08594)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This DEPDC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-38 amino acids from the N-terminal region of human DEPDC1.
Reactive Species
A08594 is reactive to DEPDC1 in Human
Calculated molecular weight
93.0 kDa
Background of DEPDC1
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08594 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
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DEPDC1 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane). This demonstrates the DEPDC1 antibody detected the DEPDC1 protein (arrow).
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All lanes : Anti-DEPDC1 Antibody (N-term) at 1:1000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 93kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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DEPDC1 Antibody (N-term) (Cat. #A08594) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEPDC1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Specific Publications For Anti-DEPDC1 Antibody (N-term) (A08594)
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