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Product Info Summary
| SKU: | A03811 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-DEPTOR Antibody
SKU/Catalog Number
A03811
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DEPTOR Antibody (Catalog # A03811). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DEPTOR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-DEPTOR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03811)
Host
Rabbit
Contents
DEPTOR Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR. The immunogen is located within amino acids 220 - 270 of DEPTOR.
Cross-reactivity
DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms.
Reactive Species
A03811 is reactive to DEPTOR in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.3 kDa
Background of DEPTOR
DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03811 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of DEPTOR in mouse liver tissue with DEPTOR antibody at 5 μg/mL.
Specific Publications For Anti-DEPTOR Antibody (A03811)
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