|Validated Species:||Human, Mouse, Rabbit, Rat|
Data & Images
|Product Name||Anti-Desmin Antibody (Monoclonal, DE-U-10)|
|Description||Mouse IgG monoclonal antibody for Desmin , desmin (DES) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Desmin Antibody (Monoclonal, DE-U-10) DE-U-10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1036)|
|Replacement Item||This antibody may replace the following items: sc-130593|sc-134338|sc-14026|sc-162734|sc-23879|sc-271677|sc-34200|sc-34201|sc-365130|sc-52326|sc-58744|sc-58745|sc-65983|sc-70961|sc-7559 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rabbit, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Desmin from pig stomach.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||53457 MW|
|Protein Function||Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. .|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Subcellular Localization||Cytoplasm, myofibril, sarcomere, Z line . Cytoplasm . Cell membrane, sarcolemma . Localizes in the intercalated disks which occur at the Z line of cardiomyocytes. .|
|Research Areas|||immunology|innate immunity|complement|classical pathway| immunology|alternative pathway||
Background for Desmin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Desmin Antibody (Monoclonal, DE-U-10) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rat, rabbit, By Heat
Western blot, 2μg/ml, Human, mouse, rat, rabbit
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Desmin Antibody (Monoclonal, DE-U-10) Images
Click the images to enlarge.
IHC(P): Rat Cardiac Muscle Tissue
All lanes: Anti Desmin (MA1036) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 53KD
Observed bind size: 53KD