Anti-Desmin Antibody (Monoclonal, DE-U-10)
Mouse IgG monoclonal antibody for Desmin , desmin (DES) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit. No cross reactivity with other proteins.
|Reactivity||Human, Mouse, Rabbit, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Desmin Antibody (Monoclonal, DE-U-10)
See all Des primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for Desmin/DES detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Desmin/DES information: Molecular Weight: 53457 MW; Subcellular Localization: Cytoplasm, myofibril, sarcomere, Z line . Cytoplasm . Cell membrane, sarcolemma . Localizes in the intercalated disks which occur at the Z line of cardiomyocytes.|
|Cite This Product||Anti-Desmin Antibody (Monoclonal, DE-U-10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1036)|
|Specificity||Anti-Desmin Antibody (Monoclonal, DE-U-10) (MA1036) reacts with Human, Mouse, Rabbit, Rat Des, in native form and recombinant. Superfamily members of Des are not reactive to MA1036.|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Immunogen||Desmin from pig stomach.|
|Reactivity||Human, Mouse, Rabbit, Rat|
Our Boster Quality Guarantee for Anti-Desmin Antibody (Monoclonal, DE-U-10) covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rat, rabbit, By Heat
Western blot, 2μg/ml, Human, mouse, rat, rabbit
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-Desmin Antibody (Monoclonal, DE-U-10) (MA1036).Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Images And Assay Conditions
Anti-Desmin antibody (monoclonal), MA1036, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Anti-Desmin antibody (monoclonal), MA1036, Western blotting
All lanes: Anti Desmin (MA1036) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 53KD
Observed bind size: 53KD
Protein Target Info (Source: Uniprot.org)
|Subcellular Localization||Cytoplasm, myofibril, sarcomere, Z line . Cytoplasm . Cell membrane, sarcolemma . Localizes in the intercalated disks which occur at the Z line of cardiomyocytes. .|
|Molecular Weight||53457 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. .|
|Research Areas||Human, Mouse, Rabbit, Rat
*You can search these to find other products in these research areas.
|Background||Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball(1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al.(1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]