|Validated Species:||Human, Mouse, Rabbit, Rat|
Data & Images
|Product Name||Anti-Desmin Antibody (Monoclonal, DE-U-10)|
|Description||Mouse IgG monoclonal antibody for Desmin , desmin (DES) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Desmin Antibody (Monoclonal, DE-U-10) DE-U-10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1036)|
|Replacement Item||This antibody may replace the following items: sc-130593|sc-134338|sc-14026|sc-162734|sc-23879|sc-271677|sc-34200|sc-34201|sc-365130|sc-52326|sc-58744|sc-58745|sc-65983|sc-70961|sc-7559 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rabbit, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Desmin from pig stomach.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||53457 MW|
|Protein Function||Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. .|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Subcellular Localization||Cytoplasm, myofibril, sarcomere, Z line . Cytoplasm . Cell membrane, sarcolemma . Localizes in the intercalated disks which occur at the Z line of cardiomyocytes. .|
|Research Areas|||immunology|innate immunity|complement|classical pathway| immunology|alternative pathway||
Background for Desmin
Anti-Desmin Antibody (Monoclonal, DE-U-10) Images
Click the images to enlarge.
IHC(P): Rat Cardiac Muscle Tissue
All lanes: Anti Desmin (MA1036) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 53KD
Observed bind size: 53KD