Click image to see more details
Product Info Summary
| SKU: | M01948-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rabbit, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Desmin Antibody (Monoclonal, 35D56)
SKU/Catalog Number
M01948-4
BM0036 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Desmin Antibody (Monoclonal, 35D56) catalog # M01948-4. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Desmin Antibody (Monoclonal, 35D56) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01948-4)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
35D56
Isotype
Mouse IgG1
Immunogen
Desmin from pig stomach.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
M01948-4 is reactive to Des in Human, Mouse, Rabbit, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
53.5 kDa
Background of Des
Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball (1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al. (1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01948-4 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, rabbit
Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rat, rabbit
Positive Control
WB: rat heart tissue, rat skeletal muscle tissue, rat C2C12 whole cell, mouse heart tissue, mouse skeletal muscle tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Desmin using anti-Desmin antibody (M01948-4).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat C2C12 whole cell lysates.
Lane 4: mouse heart tissue lysates,
Lane 5: mouse skeletal muscle tissue lysates.
After Electrophoresis, proteins were tDesminsferred to a Nitrocellulose membDesmine at 150mA for 50-90 minutes. Blocked the membDesmine with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membDesmine was incubated with mouse anti-Desmin antigen affinity purified monoclonal antibody (Catalog # M01948-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54 kDa. The expected band size for Desmin is at 54 kDa.
Specific Publications For Anti-Desmin Antibody (Monoclonal, 35D56) (M01948-4)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Desmin Antibody (Monoclonal, 35D56)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Desmin Antibody (Monoclonal, 35D56)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
