Anti-Desmin Antibody (Monoclonal, DE-U-10)

Boster Bio Anti-Desmin Antibody (Monoclonal, DE-U-10) catalog # MA1036. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat. Cited in 21 publication(s).

Product Info Summary

SKU: MA1036
Size: 100μg/vial
Reactive Species: Human, Mouse, Rabbit, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-Desmin Antibody (Monoclonal, DE-U-10)

See all Desmin products

SKU/Catalog Number

MA1036

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Desmin Antibody (Monoclonal, DE-U-10) catalog # MA1036. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Desmin Antibody (Monoclonal, DE-U-10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1036)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DE-U-10

Isotype

Mouse IgG1

Immunogen

Desmin from pig stomach.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1036 is reactive to Des in Human, Mouse, Rabbit, Rat

Applications

MA1036 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rat, rabbit, By Heat
Western blot, 2μg/ml, Human, mouse, rat, rabbit

Validation Images & Assay Conditions

Gene/Protein Information For Des (Source: Uniprot.Org, NCBI)

Uniprot ID

P48675

Gene ID

64362

Gene Name

Des

Full Name

Desmin

Weight

53.457kDa

Superfamily

intermediate filament family

Alternative Names

CMD1I; CMD1IFLJ41013; CSM1; CSM2; DES; Desmin; FLJ12025; FLJ39719; FLJ41793; intermediate filament protein Des|desmin|desmin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Des, check out the Des Infographic

Des infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Des: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1036 has been cited in 21 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Interleukin-10 promotes primary rat hepatic stellate cell senescence by upregulating the expression levels of p53 and p21

Identification of apoptosis-related microRNAs and their target genes in myocardial infarction post-transplantation with skeletal myoblasts

Liu J, Wang Y, Du W, Liu W, Liu F, Zhang L, Zhang M, Hou M, Liu K, Zhang S, Yu B. Plos One. 2013;8(3):E58883. Doi: 10.1371/Journal.Pone.0058883. Epub 2013 Mar 22. Wnt1 Inhibits Hydrogen Peroxide-Induced Apoptosis In Mouse Cardiac Stem Cells.

Wang W, Fan M, Zhang L, Liu Sh, Sun L, Wang Cy. Biomed Mater. 2009 Apr;4(2):025011. Doi: 10.1088/1748-6041/4/2/025011. Epub 2009 Mar 4. Compatibility Of Hyaluronic Acid Hydrogel And Skeletal Muscle Myoblasts.

Zhang F, Deng B, Wen J, Chen K, Liu W, Ye S, Huang H, Jiang S, Xiong Y. Biochem Biophys Res Commun. 2015 Mar 6;458(2):375-80. Doi: 10.1016/J.Bbrc.2015.01.120. Epub 2015 Jan 31. Ppar?? And Myod Are Differentially Regulated By Myostatin In Adipose-D...

Liu Hh, Li L, Chen X, Cao W, Zhang Rp, Yu Hy, Xu F, He H, Wang Jw. Cytotechnology. 2011 Aug;63(4):399-406. Doi: 10.1007/S10616-011-9356-7. Epub 2011 May 26. Characterization Of In Vitro Cultured Myoblasts Isolated From Duck (Anas Platyrhynchos) Em...

Chen S, Fang Z, Zhu Z, Deng A, Liu J, Zhang C. J Huazhong Univ Sci Technolog Med Sci. 2009 Dec;29(6):715-9. Doi: 10.1007/S11596-009-0608-0. Epub 2009 Dec 29. Protective Effect Of Sulodexide On Podocyte Injury In Adriamycin Nephropathy Rats.

Liu J, Liu Y, Xu Y, Wang M, Zhu Q, Li W, Wang X, Liu J, Ma B, Wu K. Exp Mol Pathol. 2013 Feb;94(1):79-83. Doi: 10.1016/J.Yexmp.2012.10.012. Epub 2012 Oct 23. Neurotransmitter Noradrenaline Downregulate Cytoskeletal Protein Expression Of Vsmcs.

Sondag Gr, Salihoglu S, Lababidi Sl, Crowder Dc, Moussa Fm, Abdelmagid Sm, Safadi Ff. J Cell Physiol. 2014 Jul;229(7):955-66. Doi: 10.1002/Jcp.24512. Osteoactivin Induces Transdifferentiation Of C2C12 Myoblasts Into Osteoblasts.

Zhang Sc, Zheng Yh, Yu Pp, Min Th, Yu Fx, Ye C, Xie Yk, Zhang Qy. World J Gastroenterol. 2012 Jul 28;18(28):3696-704. Doi: 10.3748/Wjg.V18.I28.3696. Lentiviral Vector-Mediated Down-Regulation Of Il-17A Receptor In Hepatic Stellate Cells Results In...

Liu Hh, Wang Jw, Yu Hy, Zhang Rp, Chen X, Jin Hb, Dai F, Li L, Xu F. Appl Microbiol Biotechnol. 2012 Jun;94(5):1255-63. Doi: 10.1007/S00253-012-3908-4. Epub 2012 Feb 18. Injection Of Duck Recombinant Follistatin Fusion Protein Into Duck Muscle Tis...

Wang Xq, Yang Wj, Yang Z, Shu G, Wang Sb, Jiang Qy, Yuan L, Wu Ts. Plos One. 2012;7(3):E32537. Doi: 10.1371/Journal.Pone.0032537. Epub 2012 Mar 13. The Differential Proliferative Ability Of Satellite Cells In Lantang And Landrace Pigs.

Yan J, Gan L, Yang H, Sun C. Mol Biol Rep. 2013 Apr;40(4):3197-202. Doi: 10.1007/S11033-012-2395-0. Epub 2012 Dec 29. The Proliferation And Differentiation Characteristics Of Co-Cultured Porcine Preadipocytes And Muscle Satellite Cells In Vitro.

Gao Cq, Zhi R,, Yang Z,, Li Hc, Yan Hc, Wang Xq. J Cell Biochem. 2015 Nov;116(11):2637-48. Doi: 10.1002/Jcb.25212. Low Dose Of Igf-I Increases Cell Size Of Skeletal Muscle Satellite Cells Via Akt/S6K Signaling Pathway.

Xu X, Qian H, Zhu W, Zhang X, Yan Y, Wang M, Xu W. J Mol Med (Berl). 2010 Nov;88(11):1181-90. Doi: 10.1007/S00109-010-0659-5. Epub 2010 Aug 10. Isolation Of Cancer Stem Cells From Transformed Human Mesenchymal Stem Cell Line F6.

Pan X, Chen Z, Huang R, Yao Y, Ma G. Plos One. 2013;8(4):E60335. Doi: 10.1371/Journal.Pone.0060335. Epub 2013 Apr 1. Transforming Growth Factor ??1 Induces The Expression Of Collagen Type I By Dna Methylation In Cardiac Fibroblasts.

Qian Q, Qian H, Zhang X, Zhu W, Yan Y, Ye S, Peng X, Li W, Xu Z, Sun L, Xu W. Stem Cells Dev. 2012 Jan;21(1):67-75. Doi: 10.1089/Scd.2010.0519. Epub 2011 Jun 1. 5-Azacytidine Induces Cardiac Differentiation Of Human Umbilical Cord-Derived Mesenchy...

Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.

Longxi P, Buwu F, Yuan W, Sinan G. Plos One. 2011;6(10):E26500. Doi: 10.1371/Journal.Pone.0026500. Epub 2011 Oct 28. Expression Of P53 In The Effects Of Artesunate On Induction Of Apoptosis And Inhibition Of Proliferation In Rat Primary Hepatic St...

Wu R, Gu B, Zhao X, Tan Z, Chen L, Zhu J, Zhang M. Hum Cell. 2013 Mar;26(1):19-27. Doi: 10.1007/S13577-011-0022-3. Epub 2011 Jun 15. Derivation Of Multipotent Nestin(+)/Cd271 (-)/Stro-1 (-) Mesenchymal-Like Precursors From Human Embryonic Stem Cel...

Wei L, Yang J, Wang M, Xu Sn, Liang Hm, Zhou Q. Int J Mol Med. 2014 Nov;34(5):1257-67. Doi: 10.3892/Ijmm.2014.1905. Epub 2014 Aug 19. Sodium Ferulate Lowers Portal Pressure In Rats With Secondary Biliary Cirrhosis Through The Rhoa/Rho-Kinase Signa...

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16 Customer Q&As for Anti-Desmin Antibody (Monoclonal, DE-U-10)

Question

Do you have a BSA free version of anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 available?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-14

Question

Is this MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) reactive to the isotypes of DES?

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

The immunogen of MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) is Desmin from pig stomach. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-06

Question

Is a blocking peptide available for product anti-Desmin antibody (Monoclonal, DE-U-10) (MA1036)?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

We do provide the blocking peptide for product anti-Desmin antibody (Monoclonal, DE-U-10) (MA1036). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-24

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagogastric junction muscularis propria using anti-Desmin antibody (Monoclonal, DE-U-10) MA1036. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-25

Question

I was wanting to use to test anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 on human esophagogastric junction muscularis propria for research purposes, then I may be interested in using anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

R. Edwards

Verified customer

Asked: 2019-08-20

Answer

The products we sell, including anti-Desmin antibody (Monoclonal, DE-U-10) MA1036, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-20

Question

I would like using your anti-Desmin antibody (Monoclonal, DE-U-10) for r-rno-390522; striated muscle contraction studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

I appreciate your inquiry. This MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) is tested on rat skeletal muscle tissue, cardiac muscle tissue, tissue lysate, mouse skeletal muscle tissue. It is guaranteed to work for IHC, WB in human, mouse, rabbit, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-27

Question

My team were happy with the WB result of your anti-Desmin antibody (Monoclonal, DE-U-10). However we have observed positive staining in muscle myofibril using this antibody. Is that expected? Could you tell me where is DES supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

From literature, muscle does express DES. Generally DES expresses in cytoplasm, myofibril, sarcomere, z line. Regarding which tissues have DES expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 8792816, 15489334
Skeletal muscle, Pubmed ID: 10545598

Boster Scientific Support

Answered: 2019-06-21

Question

Would anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 work for WB with esophagogastric junction muscularis propria?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

According to the expression profile of esophagogastric junction muscularis propria, DES is highly expressed in esophagogastric junction muscularis propria. So, it is likely that anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 will work for WB with esophagogastric junction muscularis propria.

Boster Scientific Support

Answered: 2019-06-20

Question

I see that the anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-04-22

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-22

Question

See attached the WB image, lot number and protocol we used for esophagogastric junction muscularis propria using anti-Desmin antibody (Monoclonal, DE-U-10) MA1036. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-02-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-01

Question

My question regarding product MA1036, anti-Desmin antibody (Monoclonal, DE-U-10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-01-15

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1036 anti-Desmin antibody (Monoclonal, DE-U-10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-15

Question

We are currently using anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rabbit, rat. Is it true that the antibody can work on feline tissues as well?

L. Miller

Verified customer

Asked: 2017-09-01

Answer

The anti-Desmin antibody (Monoclonal, DE-U-10) (MA1036) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-01

Question

I was wanting to use your anti-Desmin antibody (Monoclonal, DE-U-10) for WB for human esophagogastric junction muscularis propria on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human esophagogastric junction muscularis propria identification?

Verified Customer

Verified customer

Asked: 2017-08-21

Answer

It shows on the product datasheet, MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) has been tested for IHC, WB on human, mouse, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in human esophagogastric junction muscularis propria in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-21

Question

Our lab used your anti-Desmin antibody (Monoclonal, DE-U-10) for IHC on muscle in a previous project. I am using rabbit, and We intend to use the antibody for WB next. My lab would like examining muscle as well as esophagogastric junction muscularis propria in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

K. Carter

Verified customer

Asked: 2016-11-23

Answer

I viewed the website and datasheets of our anti-Desmin antibody (Monoclonal, DE-U-10) and I see that MA1036 has been tested on rabbit in both IHC and WB. Thus MA1036 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rabbit even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-11-23

Question

We have been able to see staining in rat liver. Any tips? Is anti-Desmin antibody (Monoclonal, DE-U-10) supposed to stain liver positively?

K. Li

Verified customer

Asked: 2015-11-02

Answer

From what I have seen in literature liver does express DES. From what I have seen in Uniprot.org, DES is expressed in esophagogastric junction muscularis propria, muscle, skeletal muscle, liver, among other tissues. Regarding which tissues have DES expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 8792816, 15489334
Skeletal muscle, Pubmed ID: 10545598

Boster Scientific Support

Answered: 2015-11-02

Question

Would MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

E. Zhang

Verified customer

Asked: 2013-11-13

Answer

It shows on the product datasheet, MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-11-13

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MA1036
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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