Anti-Desmin Antibody (Monoclonal, DE-U-10)

SKU MA1036
Size 100μg/vial
Reactivity Human, Mouse, Rabbit, Rat
Clonality Monoclonal
Clone Number DE-U-10
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Desmin Antibody (Monoclonal, DE-U-10)
SKU/Catalog Number MA1036
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Desmin , desmin (DES) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Desmin Antibody (Monoclonal, DE-U-10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1036)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Desmin from pig stomach.
Reactivity Human, Mouse, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rat, rabbit, By Heat
Western blot, 2μg/ml, Human, mouse, rat, rabbit

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

Anti-Desmin antibody (monoclonal), MA1036, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue

Anti-Desmin antibody (monoclonal), MA1036, Western blotting
All lanes: Anti Desmin (MA1036) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 53KD
Observed bind size: 53KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P48675
Gene Name Des
Protein Name Desmin
Alternative Names Desmin;Des;
Subcellular Localization Cytoplasm, myofibril, sarcomere, Z line . Cytoplasm . Cell membrane, sarcolemma . Localizes in the intercalated disks which occur at the Z line of cardiomyocytes. .
Molecular Weight 53457 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. .
Research Areas Human, Mouse, Rabbit, Rat

*You can search these to find other products in these research areas.
Background Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball(1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al.(1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.

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Monoclonal clone# G2 antibody for Desmin/DES detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Desmin/DES information: Molecular Weight: 53457 MW; Subcellular Localization: Cytoplasm, myofibril, sarcomere, Z line . Cytoplasm . Cell membrane, sarcolemma . Localizes in the intercalated disks which occur at the Z line of cardiomyocytes.
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In stock
Order Product
MA1036
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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Publications

Notch signaling may negatively regulate neonatal rat cardiac fibroblast-myofibroblast transformation
Interleukin-10 promotes primary rat hepatic stellate cell senescence by upregulating the expression levels of p53 and p21
Identification of apoptosis-related microRNAs and their target genes in myocardial infarction post-transplantation with skeletal myoblasts
Liu J, Wang Y, Du W, Liu W, Liu F, Zhang L, Zhang M, Hou M, Liu K, Zhang S, Yu B. Plos One. 2013;8(3):E58883. Doi: 10.1371/Journal.Pone.0058883. Epub 2013 Mar 22. Wnt1 Inhibits Hydrogen Peroxide-Induced Apoptosis In Mouse Cardiac Stem Cells.
Wang W, Fan M, Zhang L, Liu Sh, Sun L, Wang Cy. Biomed Mater. 2009 Apr;4(2):025011. Doi: 10.1088/1748-6041/4/2/025011. Epub 2009 Mar 4. Compatibility Of Hyaluronic Acid Hydrogel And Skeletal Muscle Myoblasts.
Zhang F, Deng B, Wen J, Chen K, Liu W, Ye S, Huang H, Jiang S, Xiong Y. Biochem Biophys Res Commun. 2015 Mar 6;458(2):375-80. Doi: 10.1016/J.Bbrc.2015.01.120. Epub 2015 Jan 31. Ppar?? And Myod Are Differentially Regulated By Myostatin In Adipose-D...
Liu Hh, Li L, Chen X, Cao W, Zhang Rp, Yu Hy, Xu F, He H, Wang Jw. Cytotechnology. 2011 Aug;63(4):399-406. Doi: 10.1007/S10616-011-9356-7. Epub 2011 May 26. Characterization Of In Vitro Cultured Myoblasts Isolated From Duck (Anas Platyrhynchos) Em...
Chen S, Fang Z, Zhu Z, Deng A, Liu J, Zhang C. J Huazhong Univ Sci Technolog Med Sci. 2009 Dec;29(6):715-9. Doi: 10.1007/S11596-009-0608-0. Epub 2009 Dec 29. Protective Effect Of Sulodexide On Podocyte Injury In Adriamycin Nephropathy Rats.
Liu J, Liu Y, Xu Y, Wang M, Zhu Q, Li W, Wang X, Liu J, Ma B, Wu K. Exp Mol Pathol. 2013 Feb;94(1):79-83. Doi: 10.1016/J.Yexmp.2012.10.012. Epub 2012 Oct 23. Neurotransmitter Noradrenaline Downregulate Cytoskeletal Protein Expression Of Vsmcs.
Sondag Gr, Salihoglu S, Lababidi Sl, Crowder Dc, Moussa Fm, Abdelmagid Sm, Safadi Ff. J Cell Physiol. 2014 Jul;229(7):955-66. Doi: 10.1002/Jcp.24512. Osteoactivin Induces Transdifferentiation Of C2C12 Myoblasts Into Osteoblasts.
Zhang Sc, Zheng Yh, Yu Pp, Min Th, Yu Fx, Ye C, Xie Yk, Zhang Qy. World J Gastroenterol. 2012 Jul 28;18(28):3696-704. Doi: 10.3748/Wjg.V18.I28.3696. Lentiviral Vector-Mediated Down-Regulation Of Il-17A Receptor In Hepatic Stellate Cells Results In...
Liu Hh, Wang Jw, Yu Hy, Zhang Rp, Chen X, Jin Hb, Dai F, Li L, Xu F. Appl Microbiol Biotechnol. 2012 Jun;94(5):1255-63. Doi: 10.1007/S00253-012-3908-4. Epub 2012 Feb 18. Injection Of Duck Recombinant Follistatin Fusion Protein Into Duck Muscle Tis...
Wang Xq, Yang Wj, Yang Z, Shu G, Wang Sb, Jiang Qy, Yuan L, Wu Ts. Plos One. 2012;7(3):E32537. Doi: 10.1371/Journal.Pone.0032537. Epub 2012 Mar 13. The Differential Proliferative Ability Of Satellite Cells In Lantang And Landrace Pigs.
Yan J, Gan L, Yang H, Sun C. Mol Biol Rep. 2013 Apr;40(4):3197-202. Doi: 10.1007/S11033-012-2395-0. Epub 2012 Dec 29. The Proliferation And Differentiation Characteristics Of Co-Cultured Porcine Preadipocytes And Muscle Satellite Cells In Vitro.
Gao Cq, Zhi R,, Yang Z,, Li Hc, Yan Hc, Wang Xq. J Cell Biochem. 2015 Nov;116(11):2637-48. Doi: 10.1002/Jcb.25212. Low Dose Of Igf-I Increases Cell Size Of Skeletal Muscle Satellite Cells Via Akt/S6K Signaling Pathway.
Xu X, Qian H, Zhu W, Zhang X, Yan Y, Wang M, Xu W. J Mol Med (Berl). 2010 Nov;88(11):1181-90. Doi: 10.1007/S00109-010-0659-5. Epub 2010 Aug 10. Isolation Of Cancer Stem Cells From Transformed Human Mesenchymal Stem Cell Line F6.
Pan X, Chen Z, Huang R, Yao Y, Ma G. Plos One. 2013;8(4):E60335. Doi: 10.1371/Journal.Pone.0060335. Epub 2013 Apr 1. Transforming Growth Factor ??1 Induces The Expression Of Collagen Type I By Dna Methylation In Cardiac Fibroblasts.
Qian Q, Qian H, Zhang X, Zhu W, Yan Y, Ye S, Peng X, Li W, Xu Z, Sun L, Xu W. Stem Cells Dev. 2012 Jan;21(1):67-75. Doi: 10.1089/Scd.2010.0519. Epub 2011 Jun 1. 5-Azacytidine Induces Cardiac Differentiation Of Human Umbilical Cord-Derived Mesenchy...
Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.
Longxi P, Buwu F, Yuan W, Sinan G. Plos One. 2011;6(10):E26500. Doi: 10.1371/Journal.Pone.0026500. Epub 2011 Oct 28. Expression Of P53 In The Effects Of Artesunate On Induction Of Apoptosis And Inhibition Of Proliferation In Rat Primary Hepatic St...
Wu R, Gu B, Zhao X, Tan Z, Chen L, Zhu J, Zhang M. Hum Cell. 2013 Mar;26(1):19-27. Doi: 10.1007/S13577-011-0022-3. Epub 2011 Jun 15. Derivation Of Multipotent Nestin(+)/Cd271 (-)/Stro-1 (-) Mesenchymal-Like Precursors From Human Embryonic Stem Cel...
Wei L, Yang J, Wang M, Xu Sn, Liang Hm, Zhou Q. Int J Mol Med. 2014 Nov;34(5):1257-67. Doi: 10.3892/Ijmm.2014.1905. Epub 2014 Aug 19. Sodium Ferulate Lowers Portal Pressure In Rats With Secondary Biliary Cirrhosis Through The Rhoa/Rho-Kinase Signa...

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
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