Anti-DFF40 Dffb Antibody

Product Name

Anti-DFF40 Dffb Antibody

View all DFFB Antibodies

SKU/Catalog Number

A05374-3

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DFF40 Dffb Antibody (Catalog # A05374-3). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DFF40 Dffb Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05374-3)

Host

Rabbit

Contents

DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DFF40 antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD. The immunogen is located within amino acids 130 - 180 of DFF40.

Reactive Species

A05374-3 is reactive to DFFB in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

39.4 kDa

Background of DFFB

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05374-3 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 μg/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information DFFB

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