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Product Info Summary
| SKU: | A06354 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-DGK- alpha Antibody
SKU/Catalog Number
A06354
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DGK- alpha Antibody catalog # A06354. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DGK- alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06354)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DGKA. AA range:304-353
Reactive Species
A06354 is reactive to DGKA in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
82.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06354 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunofluorescence analysis of MCF7 cell. 1,primary Antibody was diluted at 1:100 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500 (room temperature, 50min).
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Western Blot analysis of various cells using DGK-α Polyclonal Antibody
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Western Blot analysis of Lovo cells using DGK-α Polyclonal Antibody
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Western blot analysis of lysates from Jurkat cells, using DGKA Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 30min).
Specific Publications For Anti-DGK- alpha Antibody (A06354)
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Customer Q&As
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6 Customer Q&As for Anti-DGK- alpha Antibody
Question
I see that the anti-DGK- alpha antibody A06354 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-09
Question
Would anti-DGK- alpha antibody A06354 work on feline WB with uterus?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
Our lab technicians have not validated anti-DGK- alpha antibody A06354 on feline. You can run a BLAST between feline and the immunogen sequence of anti-DGK- alpha antibody A06354 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-DGK- alpha antibody A06354. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-08
Question
I was wanting to use your anti-DGK- alpha antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
S. Jones
Verified customer
Asked: 2019-06-26
Answer
It shows on the product datasheet, A06354 anti-DGK- alpha antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-26
Question
Would A06354 anti-DGK- alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
It shows on the product datasheet, A06354 anti-DGK- alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-10
Question
We are currently using anti-DGK- alpha antibody A06354 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
A. Baker
Verified customer
Asked: 2015-11-30
Answer
The anti-DGK- alpha antibody (A06354) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-30
