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Product Info Summary
| SKU: | A02903 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Thyroxine 5-deiodinase DIO3 Antibody
SKU/Catalog Number
A02903
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Thyroxine 5-deiodinase DIO3 Antibody catalog # A02903. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thyroxine 5-deiodinase DIO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02903)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7A6
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human DIO3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02903 is reactive to DIO3 in Human, Mouse, Rat
Applications
A02903 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
33.947kDa
Background of DIO3
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of a-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Emamian ES, et al. (2004) J Neurosci. 24(7): 1561-4
Palmer, C.L. et al. (2005) Pharmacol. Rev. 57, 253-277.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using DIO3 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For DIO3 (Source: Uniprot.org, NCBI)
Gene Name
DIO3
Full Name
Thyroxine 5-deiodinase
Weight
33.947kDa
Superfamily
iodothyronine deiodinase family
Alternative Names
5DIII; D3; deiodinase, iodothyronine, type III; DIOIII; EC 1.97.1; EC 1.97.1.11; ITDI3; placental type; thyroxine deiodinase type III (selenoprotein); Type 3 DI; type 3 iodothyronine selenodeiodinase; type III iodothyronine deiodinase; type-III 5' deiodinase; Type-III 5'-deiodinase DIO3 5DIII, D3, DIOIII, TXDI3 iodothyronine deiodinase 3 thyroxine 5-deiodinase|deiodinase, iodothyronine type III|iodothyronine deiodinase, placental type|selenoprotein DIO3|thyroxine deiodinase type III (selenoprotein)|type 3 DI|type 3 iodothyronine selenodeiodinase|type-III 5 deiodinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DIO3, check out the DIO3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DIO3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thyroxine 5-deiodinase DIO3 Antibody (A02903)
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No publications found for A02903
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-Thyroxine 5-deiodinase DIO3 Antibody
Question
Will anti-DIO3 antibody A02903 work on zebrafish WB with duodenum?
V. Parker
Verified customer
Asked: 2020-02-05
Answer
Our lab technicians have not tested anti-DIO3 antibody A02903 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-DIO3 antibody A02903 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish duodenum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-05
Question
We are currently using anti-DIO3 antibody A02903 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
J. Kulkarni
Verified customer
Asked: 2016-06-01
Answer
The anti-DIO3 antibody (A02903) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-01
Question
Can you help my question with product A02903, anti-DIO3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Krishna
Verified customer
Asked: 2016-02-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02903 anti-DIO3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-02-09
Question
I was wanting to use your anti-DIO3 antibody for WB for mouse tendon on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse tendon identification?
F. Yang
Verified customer
Asked: 2014-01-30
Answer
You can see on the product datasheet, A02903 anti-DIO3 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse tendon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-30
