Anti-Delta-like protein 3 DLL3 Antibody

DLL3 antibody

Boster Bio Anti-Delta-like protein 3 DLL3 Antibody catalog # PA1912. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1912
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Delta-like protein 3 DLL3 Antibody

View all DLL3 Antibodies

SKU/Catalog Number

PA1912

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Delta-like protein 3 DLL3 Antibody catalog # PA1912. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Delta-like protein 3 DLL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1912)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DLL3 (599-618aa RAGQRQHLLFPYPSSILSVK).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1912 is reactive to DLL3 in Human

Applications

PA1912 is guaranteed for WB Boster Guarantee

Background of DLL3

DLL3 (DELTA-LIKE 3) also known as DELTA, DROSOPHILA, HOMOLOG OF, is a protein which in humans is encoded by the DLL3 gene. This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutation in the mouse delta-like 3 gene (Dll3), which is homologous to the Notch-ligand delta in Drosophila, results in the mouse 'pudgy' phenotype. The human DLL3 gene was identified within a critical interval, mapped in 2 consanguineous Arab-Israeli and Pakistani SCDO1 pedigrees, of 7.8 cM at 19q13.1-q13.3 between D19S570 and D19S908 (Bulman et al., 2000). Dunwoodie et al. (1997) presented results suggesting that mouse Dll3 may complement the function of other delta homologs during early pattern formation in the mouse embryo. In humans, the fact that mutations in genes required for oscillation, such as DLL3, result in abnormal segmentation of the vertebral column suggests that the segmentation clock also acts during human embryonic development. This residue is highly conserved in Delta proteins from Drosophila to humans, and the substitution of a charged polar for a nonpolar residue may disrupt the conformation of the DLL3 protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For DLL3 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9NYJ7

Gene ID

10683

Gene Name

DLL3

Full Name

Delta-like protein 3

Weight

64.618kDa

Alternative Names

delta (Drosophila)-like 3; Delta3; delta-like 3 (Drosophila); delta-like protein 3; DLL3; Drosophila Delta homolog 3; Pudgy; SCDO1; SCDO1delta3 DLL3 SCDO1 delta like canonical Notch ligand 3 delta-like protein 3|delta-like 3|delta3|drosophila Delta homolog 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DLL3, check out the DLL3 Infographic

DLL3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DLL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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16 Customer Q&As for Anti-Delta-like protein 3 DLL3 Antibody

Question

We have been able to see staining in human brain. What should we do? Is anti-DLL3 antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

Based on literature brain does express DLL3. Based on Uniprot.org, DLL3 is expressed in nucleus accumbens, brain, among other tissues. Regarding which tissues have DLL3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-04-14

Question

Our team were well pleased with the WB result of your anti-DLL3 antibody. However we have been able to see positive staining in nucleus accumbens membrane using this antibody. Is that expected? Could you tell me where is DLL3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

Based on literature, nucleus accumbens does express DLL3. Generally DLL3 expresses in membrane. Regarding which tissues have DLL3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-04-10

Question

I was wanting to use your anti-DLL3 antibody for WB for human nucleus accumbens on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human nucleus accumbens identification?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

It shows on the product datasheet, PA1912 anti-DLL3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human nucleus accumbens in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-27

Question

Will anti-DLL3 antibody PA1912 work for WB with nucleus accumbens?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

According to the expression profile of nucleus accumbens, DLL3 is highly expressed in nucleus accumbens. So, it is likely that anti-DLL3 antibody PA1912 will work for WB with nucleus accumbens.

Boster Scientific Support

Answered: 2020-01-31

Question

I was wanting to use using your anti-DLL3 antibody for notch signaling pathway studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

Thank you for your inquiry. This PA1912 anti-DLL3 antibody is validated on rat brain tissue, mouse brain, human hek293 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-11

Question

Would PA1912 anti-DLL3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

It shows on the product datasheet, PA1912 anti-DLL3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-16

Question

Is a blocking peptide available for product anti-DLL3 antibody (PA1912)?

H. Walker

Verified customer

Asked: 2019-06-20

Answer

We do provide the blocking peptide for product anti-DLL3 antibody (PA1912). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-20

Question

Would anti-DLL3 antibody PA1912 work on primate WB with nucleus accumbens?

Verified Customer

Verified customer

Asked: 2019-01-28

Answer

Our lab technicians have not validated anti-DLL3 antibody PA1912 on primate. You can run a BLAST between primate and the immunogen sequence of anti-DLL3 antibody PA1912 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate nucleus accumbens in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-28

Question

I see that the anti-DLL3 antibody PA1912 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-12-12

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-12-12

Question

Do you have a BSA free version of anti-DLL3 antibody PA1912 available?

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-DLL3 antibody PA1912 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-01-09

Question

Is this PA1912 anti-DLL3 antibody reactive to the isotypes of DLL3?

Verified Customer

Verified customer

Asked: 2017-07-31

Answer

The immunogen of PA1912 anti-DLL3 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human DLL3(599-618aa RAGQRQHLLFPYPSSILSVK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-07-31

Question

See attached the WB image, lot number and protocol we used for nucleus accumbens using anti-DLL3 antibody PA1912. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-07-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-13

Question

I have a question about product PA1912, anti-DLL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Taylor

Verified customer

Asked: 2017-02-06

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1912 anti-DLL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-02-06

Question

We need to test anti-DLL3 antibody PA1912 on human nucleus accumbens for research purposes, then I may be interested in using anti-DLL3 antibody PA1912 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Mangal

Verified customer

Asked: 2015-02-05

Answer

The products we sell, including anti-DLL3 antibody PA1912, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-02-05

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for nucleus accumbens using anti-DLL3 antibody PA1912. Let me know if you need anything else.

K. Bhatt

Verified customer

Asked: 2013-10-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-10-22

Question

We are currently using anti-DLL3 antibody PA1912 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?

M. Yang

Verified customer

Asked: 2013-06-03

Answer

The anti-DLL3 antibody (PA1912) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-06-03

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Total: $315

SKU:PA1912

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PA1912
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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