Overview
| Product Name |
Anti-DLL4 Antibody Picoband™
See more DLL4 products |
| Catalog# |
A00875-1 |
| Pack Size |
100μg/vial |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-DLL4 Antibody Picoband™ catalog # A00875-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody. |
| Cite This Product |
Anti-DLL4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00875-1)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E.coli-derived human DLL4 recombinant protein (Position: T114-D203). Human DLL4 shares 76.7% and 78.9% amino acid (aa) sequence identity with mouse and rat DLL4, respectively. |
| Cross Reactivity |
No cross reactivity with other proteins. |
Gene/Protein Basic Information For DLL4 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9NR61 |
|---|
| NCBI Gene Id | 54567 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | DLL4 |
|---|
| Protein Name | Delta-like protein 4 |
|---|
| Alternative Names | DLL4|Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4 |
|---|
| Molecular Weight | 74605 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on DLL4, check out the DLL4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for DLL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the DLL4 infographic
Our Boster Quality Guarantee for Anti-DLL4 Antibody Picoband™ covers its use in the following applications.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Western blot analysis of DLL4 using anti-DLL4 antibody (A00875-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL4 antigen affinity purified polyclonal antibody (Catalog # A00875-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL4 at approximately 80KD. The expected band size for DLL4 is at 75KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
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Questions and answers from customers related to A00875-1 Anti-DLL4 Antibody Picoband™
15 Related Questions
Question
I am interested in using your anti-DLL4 antibody for negative regulation of endothelial cell migration studies. Has this antibody been tested with western blotting on kidney tissue? We would like to see some validation images before ordering.
Verified Customer
Asked: 2020-03-02
Answer
Thank you for your inquiry. This A00875-1 anti-DLL4 antibody is validated on rat liver tissue, kidney tissue, mouse liver tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-02
Question
Is a blocking peptide available for product anti-DLL4 antibody (A00875-1)?
Verified Customer
Asked: 2020-02-11
Answer
We do provide the blocking peptide for product anti-DLL4 antibody (A00875-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-11
Question
We have observed staining in mouse placenta. What should we do? Is anti-DLL4 antibody supposed to stain placenta positively?
K. Taylor
Asked: 2020-01-31
Answer
According to literature placenta does express DLL4. According to Uniprot.org, DLL4 is expressed in heart left ventricle, brain, placenta, among other tissues. Regarding which tissues have DLL4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10837024, 11134954
Placenta, Pubmed ID: 17728344
Boster Scientific Support
Answered: 2020-01-31
Question
Will A00875-1 anti-DLL4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Asked: 2019-10-15
Answer
It shows on the product datasheet, A00875-1 anti-DLL4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart left ventricle using anti-DLL4 antibody A00875-1. Let me know if you need anything else.
K. Kulkarni
Asked: 2019-06-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-06
Question
I see that the anti-DLL4 antibody A00875-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Asked: 2019-04-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-25
Question
My colleagues were well pleased with the WB result of your anti-DLL4 antibody. However we have observed positive staining in heart left ventricle cell membrane using this antibody. Is that expected? Could you tell me where is DLL4 supposed to be expressed?
Verified Customer
Asked: 2018-12-24
Answer
Based on literature, heart left ventricle does express DLL4. Generally DLL4 expresses in cell membrane. Regarding which tissues have DLL4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10837024, 11134954
Placenta, Pubmed ID: 17728344
Boster Scientific Support
Answered: 2018-12-24
Question
Is this A00875-1 anti-DLL4 antibody reactive to the isotypes of DLL4?
Verified Customer
Asked: 2018-09-20
Answer
The immunogen of A00875-1 anti-DLL4 antibody is E.coli-derived human DLL4 recombinant protein (Position: T114-D203). Human DLL4 shares 76.7% and 78.9% amino acid (aa) sequence identity with mouse and rat DLL4, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-20
Question
I was wanting to use your anti-DLL4 antibody for WB for rat heart left ventricle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat heart left ventricle identification?
J. Thomas
Asked: 2018-07-31
Answer
You can see on the product datasheet, A00875-1 anti-DLL4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart left ventricle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-31
Question
We are currently using anti-DLL4 antibody A00875-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Answer
The anti-DLL4 antibody (A00875-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-12
Question
I was wanting to use to test anti-DLL4 antibody A00875-1 on rat heart left ventricle for research purposes, then I may be interested in using anti-DLL4 antibody A00875-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Jones
Asked: 2015-04-17
Answer
The products we sell, including anti-DLL4 antibody A00875-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-17
Question
I have attached the WB image, lot number and protocol we used for heart left ventricle using anti-DLL4 antibody A00875-1. Please let me know if you require anything else.
J. Jones
Asked: 2014-10-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-23
Question
I have a question about product A00875-1, anti-DLL4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Dhar
Asked: 2014-09-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00875-1 anti-DLL4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-02
Question
Is there a BSA free version of anti-DLL4 antibody A00875-1 available?
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DLL4 antibody A00875-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-08-13
Question
Will anti-DLL4 antibody A00875-1 work for WB with heart left ventricle?
M. Kulkarni
Asked: 2013-12-05
Answer
According to the expression profile of heart left ventricle, DLL4 is highly expressed in heart left ventricle. So, it is likely that anti-DLL4 antibody A00875-1 will work for WB with heart left ventricle.
Boster Scientific Support
Answered: 2013-12-05
