Anti-DLL4 Antibody Picoband™

Product Name

Anti-DLL4 Antibody Picoband™

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SKU/Catalog Number

A00875-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DLL4 Antibody Picoband™ catalog # A00875-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DLL4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00875-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Immunogen

E.coli-derived human DLL4 recombinant protein (Position: T114-D203). Human DLL4 shares 76.7% and 78.9% amino acid (aa) sequence identity with mouse and rat DLL4, respectively.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00875-1 is reactive to DLL4 in Human, Mouse, Rat

Applications

A00875-1 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of DLL4 using anti-DLL4 antibody (A00875-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL4 antigen affinity purified polyclonal antibody (Catalog # A00875-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL4 at approximately 80KD. The expected band size for DLL4 is at 75KD.

Gene/Protein Information For DLL4 (Source: Uniprot.Org, NCBI)

Alternative Names

Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4 DLL4 AOS6, delta4, hdelta2 delta like canonical Notch ligand 4 delta-like protein 4|delta 4|delta ligand 4|delta-like 4 homolog|delta-like 4 protein|drosophila Delta homolog 4|notch ligand DLL4|notch ligand delta-2

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