Anti-DLL4 Antibody Picoband™

Boster Bio Anti-DLL4 Antibody Picoband™ catalog # A00875-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00875-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-DLL4 Antibody Picoband™

See all DLL4 products

SKU/Catalog Number

A00875-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DLL4 Antibody Picoband™ catalog # A00875-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DLL4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00875-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human DLL4 recombinant protein (Position: T114-D203). Human DLL4 shares 76.7% and 78.9% amino acid (aa) sequence identity with mouse and rat DLL4, respectively.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00875-1 is reactive to DLL4 in Human, Mouse, Rat

Applications

A00875-1 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For DLL4 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NR61

Gene ID

54567

Gene Name

DLL4

Full Name

Delta-like protein 4

Weight

74.605kDa

Alternative Names

Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4 DLL4 AOS6, delta4, hdelta2 delta like canonical Notch ligand 4 delta-like protein 4|delta 4|delta ligand 4|delta-like 4 homolog|delta-like 4 protein|drosophila Delta homolog 4|notch ligand DLL4|notch ligand delta-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DLL4, check out the DLL4 Infographic

DLL4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DLL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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15 Customer Q&As for Anti-DLL4 Antibody Picoband™

Question

I am interested in using your anti-DLL4 antibody for negative regulation of endothelial cell migration studies. Has this antibody been tested with western blotting on kidney tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

Thank you for your inquiry. This A00875-1 anti-DLL4 antibody is validated on rat liver tissue, kidney tissue, mouse liver tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-02

Question

Is a blocking peptide available for product anti-DLL4 antibody (A00875-1)?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

We do provide the blocking peptide for product anti-DLL4 antibody (A00875-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-11

Question

We have observed staining in mouse placenta. What should we do? Is anti-DLL4 antibody supposed to stain placenta positively?

K. Taylor

Verified customer

Asked: 2020-01-31

Answer

According to literature placenta does express DLL4. According to Uniprot.org, DLL4 is expressed in heart left ventricle, brain, placenta, among other tissues. Regarding which tissues have DLL4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10837024, 11134954
Placenta, Pubmed ID: 17728344

Boster Scientific Support

Answered: 2020-01-31

Question

Will A00875-1 anti-DLL4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

It shows on the product datasheet, A00875-1 anti-DLL4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-15

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart left ventricle using anti-DLL4 antibody A00875-1. Let me know if you need anything else.

K. Kulkarni

Verified customer

Asked: 2019-06-06

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-06

Question

I see that the anti-DLL4 antibody A00875-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-04-25

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-25

Question

My colleagues were well pleased with the WB result of your anti-DLL4 antibody. However we have observed positive staining in heart left ventricle cell membrane using this antibody. Is that expected? Could you tell me where is DLL4 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-12-24

Answer

Based on literature, heart left ventricle does express DLL4. Generally DLL4 expresses in cell membrane. Regarding which tissues have DLL4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10837024, 11134954
Placenta, Pubmed ID: 17728344

Boster Scientific Support

Answered: 2018-12-24

Question

Is this A00875-1 anti-DLL4 antibody reactive to the isotypes of DLL4?

Verified Customer

Verified customer

Asked: 2018-09-20

Answer

The immunogen of A00875-1 anti-DLL4 antibody is E.coli-derived human DLL4 recombinant protein (Position: T114-D203). Human DLL4 shares 76.7% and 78.9% amino acid (aa) sequence identity with mouse and rat DLL4, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-20

Question

I was wanting to use your anti-DLL4 antibody for WB for rat heart left ventricle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat heart left ventricle identification?

J. Thomas

Verified customer

Asked: 2018-07-31

Answer

You can see on the product datasheet, A00875-1 anti-DLL4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart left ventricle in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-31

Question

We are currently using anti-DLL4 antibody A00875-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

J. Li

Verified customer

Asked: 2016-08-12

Answer

The anti-DLL4 antibody (A00875-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-08-12

Question

I was wanting to use to test anti-DLL4 antibody A00875-1 on rat heart left ventricle for research purposes, then I may be interested in using anti-DLL4 antibody A00875-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Jones

Verified customer

Asked: 2015-04-17

Answer

The products we sell, including anti-DLL4 antibody A00875-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-04-17

Question

I have attached the WB image, lot number and protocol we used for heart left ventricle using anti-DLL4 antibody A00875-1. Please let me know if you require anything else.

J. Jones

Verified customer

Asked: 2014-10-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-23

Question

I have a question about product A00875-1, anti-DLL4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

F. Dhar

Verified customer

Asked: 2014-09-02

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00875-1 anti-DLL4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-09-02

Question

Is there a BSA free version of anti-DLL4 antibody A00875-1 available?

T. Wu

Verified customer

Asked: 2014-08-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DLL4 antibody A00875-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-08-13

Question

Will anti-DLL4 antibody A00875-1 work for WB with heart left ventricle?

M. Kulkarni

Verified customer

Asked: 2013-12-05

Answer

According to the expression profile of heart left ventricle, DLL4 is highly expressed in heart left ventricle. So, it is likely that anti-DLL4 antibody A00875-1 will work for WB with heart left ventricle.

Boster Scientific Support

Answered: 2013-12-05

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A00875-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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