Product Info Summary
SKU: | A02588-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DNA-binding protein SATB2 SATB2 Antibody
SKU/Catalog Number
A02588-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DNA-binding protein SATB2 SATB2 Antibody catalog # A02588-1. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA-binding protein SATB2 SATB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02588-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PC-PLD2 around the phosphorylation site of Y169.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02588-1 is reactive to SATB2 in Human, Mouse, Rat
Applications
A02588-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
82.555kDa
Background of SATB2
Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (). Recruits histone deacetylases and DNA methyltransferases.
Hendrich B., Mol. Cell. Biol. 18:6538-6547(1998).
Grimwood J., Nature 428:529-535(2004).
Tatematsu K., Genes Cells 5:677-688(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC/IF: 1:50-1:100
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of SATB2 using Anti-DNA-binding protein SATB2 SATB2 Antibody (A02588-1).
ICC staining SATB2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-DNA-binding protein SATB2 SATB2 Antibody A02588-1
Western blot analysis of SATB2 on THP-1 cell using anti-SATB2 antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SATB2 (Source: Uniprot.org, NCBI)
Gene Name
SATB2
Full Name
DNA-binding protein SATB2
Weight
82.555kDa
Superfamily
CUT homeobox family
Alternative Names
DNA-binding protein SATB2; FLJ32076; KIAA1034FLJ21474; MGC119474; MGC119477; SATB family member 2; SATB homeobox 2; Special AT-rich sequence-binding protein 2 SATB2 GLSS SATB homeobox 2 DNA-binding protein SATB2|SATB family member 2|special AT-rich sequence-binding protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SATB2, check out the SATB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SATB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA-binding protein SATB2 SATB2 Antibody (A02588-1)
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No publications found for A02588-1
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