Anti-DNA-binding protein SATB2 SATB2 Antibody

Product Name

Anti-DNA-binding protein SATB2 SATB2 Antibody

View all SATB2 Antibodies

SKU/Catalog Number

A02588

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DNA-binding protein SATB2 SATB2 Antibody (Catalog # A02588). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SATB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DNA-binding protein SATB2 SATB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02588)

Host

Rabbit

Contents

SATB2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SATB2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of the human SATB2. The immunogen is located within the first 50 amino acids of SATB2.

Cross-reactivity

At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.

Reactive Species

A02588 is reactive to SATB2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

82.6 kDa

Background of SATB2

Human special AT-rich sequence-binding protein-2 (SATB2) is a nuclear matrix/scaffold-associated region DNA-binding protein. Like its homolog SATB1, SATB2 selectively binds double-stranded, special AT-rich DNA sequences, but is expressed primarily in a subset of postmitotic, differentiating neurons in the neocortex. Mice deficient in SATB exhibit craniofacial abnormalities and defects in osteoblast differentiation and function. SATB2 also interacts with and enhances the activity of Runx2 and ATF4, two transcription factors that regulate osteoblast differentiation, indicating that SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Recent experiments have shown that SATB2 interacts with histone deacetylase 1 and metastasis-associated protein 2, two proteins that are involved in chromatin remodeling, suggesting that SATB2 may also be involved in mediating epigenetic influences during cortical development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02588 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SATB2 antibody can be used for detection of SATB2 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SATB2

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