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Product Info Summary
| SKU: | A02741 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-DNA Ligase III LIG3 Antibody
SKU/Catalog Number
A02741
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DNA Ligase III LIG3 Antibody catalog # A02741. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA Ligase III LIG3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02741)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from DNA Ligase III . at AA range: 110-190
Reactive Species
A02741 is reactive to LIG3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
112.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02741 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using DNA Ligase III Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western Blot analysis of MCF7 cells using DNA Ligase III Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-DNA Ligase III LIG3 Antibody (A02741)
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Customer Q&As
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5 Customer Q&As for Anti-DNA Ligase III LIG3 Antibody
Question
We are currently using anti-DNA Ligase III antibody A02741 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
B. Miller
Verified customer
Asked: 2020-01-16
Answer
The anti-DNA Ligase III antibody (A02741) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-16
Question
Do you have a BSA free version of anti-DNA Ligase III antibody A02741 available?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-DNA Ligase III antibody A02741 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-08
Question
Does A02741 anti-DNA Ligase III antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
You can see on the product datasheet, A02741 anti-DNA Ligase III antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-05
Question
Is a blocking peptide available for product anti-DNA Ligase III antibody (A02741)?
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
We do provide the blocking peptide for product anti-DNA Ligase III antibody (A02741). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-04-23
Question
Would anti-DNA Ligase III antibody A02741 work on canine WB with cervix carcinoma?
B. Gonzalez
Verified customer
Asked: 2018-02-07
Answer
Our lab technicians have not tested anti-DNA Ligase III antibody A02741 on canine. You can run a BLAST between canine and the immunogen sequence of anti-DNA Ligase III antibody A02741 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-07
