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- Table of Contents
DNA ligase 3 Antibodies
AND LIG3 ELISA kits, DNA ligase 3 Proteins
Many biological assays use antibodies to detect DNA ligase 3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DNA ligase 3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DNA ligase 3 antibodies...
Designing a Western blot for DNA ligase 3? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the LIG3 Western Blot GuideLIG3 Infographic by Boster Bio
All LIG3 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, WB
$370
Cat # A02741-2
100 μg/vial
Cat # A02741-2
100 μg/vial
Human
$628
Cat # LS003980
20 µg, 100 µg
Cat # LS003980
20 µg, 100 µg
LIG3 Overview
Facts about DNA ligase 3.
DNA ligase 3 (LIG3)
Isoform 3 acts as heterodimer with DNA-repair protein XRCC1 in the nucleus and may fix defective DNA strand- break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it acts as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627).
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Gene Information
| Human | |
|---|---|
| Gene Name: | LIG3 |
| Uniprot: | P49916 |
| Entrez: | 3980 |
Family
| Belongs to: |
|---|
| ATP-dependent DNA ligase family |
Alternative Names
DNA ligase 3; DNA ligase III; EC 6.5.1.1; LIG2; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 3
Molecular Weight
Mass (kDA):
112.907 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 17q12 |
| Sequence: | 17; NC_000017.11 (34980498..35009743) |
Tissue Specificity
Testis, thymus, prostate and heart.
Subcellular Localizations
[Isoform 1]: Mitochondrion. Contains an N-terminal mitochondrial transit peptide.; [Isoform 2]: Mitochondrion. Contains an N-terminal mitochondrial transit peptide.; [Isoform 3]: Nucleus. Lacks the N-terminal mitochondrial transit peptide.; [Isoform 4]: Nucleus. Lacks the N-terminal mitochondrial transit peptide.
Diseases
- Malignant Neoplasms
- Carcinoma
- Neoplasms
- Malignant Neoplasm Of Breast
- Malignant Neoplasm Of Urinary Bladder
- Mammary Neoplasms
- Bladder Neoplasm
- Radiation Injury
- Adenocarcinoma
- Genomic Instability
- Diabetes Mellitus
- Cancer Of Nasopharynx
- Chromosomal Translocation
Interacting Proteins
- MAX
Pathways
- Dna Repair
- Dna Replication
- Cell Cycle
- Cell Adhesion
- Base-excision Repair
- Dna Alkylation
- Developmental Process
- Cellular Homeostasis
- M Phase
- V(d)j Recombination
- Spermatogenesis
- Double-strand Break Repair
- Cytokinesis
PTMs
- Phosphorylation
Research Areas
- Base Excision Repair
- Cancer
- DNA Repair
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/LIG3
bosterbio.com/bosterbio-gene-info-cards/LIG3
References
PMID: 7760816 by Wei Y.-F., et al. Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination.
PMID: 7565692 by Chen J., et al. Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination.