Anti-DNA Polymerase lambda/POLL Antibody Picoband™

Product Name

Anti-DNA Polymerase lambda/POLL Antibody Picoband™

View all DNA Polymerase lambda Antibodies

SKU/Catalog Number

A00959-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DNA Polymerase lambda/POLL Antibody Picoband™ catalog # A00959-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DNA Polymerase lambda/POLL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00959-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human DNA Polymerase lambda recombinant protein (Position: K307-N407).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00959-2 is reactive to POLL in Human, Mouse, Rat

Applications

A00959-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

63.482kDa

Background of DNA Polymerase lambda

DNA polymerase lambda, also known as POLL, is a protein found in eukaryotes. In humans, it is encoded by the POLLA gene. POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, suggesting that POLL participates in short- and long-patch base excision repair. POLL is widely expressed, with abundant expression in pachytene spermatocytes of testis and in ovary.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of DNA Polymerase lambda using anti-DNA Polymerase lambda antibody (A00959-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase lambda antigen affinity purified polyclonal antibody (Catalog # A00959-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase lambda at approximately 70KD. The expected band size for DNA Polymerase lambda is at 63KD.

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Figure 2. Flow Cytometry analysis of U20S cells using anti-DNA Polymerase lambda antibody (A00959-2).
Overlay histogram showing U20S cells stained with A00959-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNA Polymerase lambda Antibody (A00959-2,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For POLL (Source: Uniprot.org, NCBI)

Alternative Names

BETAN; DNA polymerase beta-2; DNA polymerase beta-N; DNA polymerase kappa; DNA polymerase lambda; EC 2.7.7.7; EC 4.2.99.-; FLJ46002; Pol beta2; Pol Lambda; POLKAPPA; polymerase (DNA directed), lambda POLL BETAN, POLKAPPA DNA polymerase lambda DNA polymerase lambda|DNA polymerase beta-2|DNA polymerase beta-N|DNA polymerase kappa|polymerase (DNA directed), lambda|polymerase (DNA) lambda

For more info on POLL, check out the POLL Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for POLL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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