Click image to see more details
Product Info Summary
| SKU: | A02205 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-DNA Polymerase thet POLQ Antibody
SKU/Catalog Number
A02205
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DNA Polymerase thet POLQ Antibody (Catalog# A02205). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA Polymerase thet POLQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02205)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human POLQ. Immunogen sequence location: 181-230
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02205 is reactive to POLQ in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
289.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02205 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DNA Polymerase thet Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-DNA Polymerase thet POLQ Antibody (A02205)
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Customer Q&As
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6 Customer Q&As for Anti-DNA Polymerase thet POLQ Antibody
Question
I see that the anti-DNA Polymerase thet antibody A02205 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-06
Question
Does anti-DNA Polymerase thet antibody A02205 work on canine IHC with secondary oocyte?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Our lab technicians have not tested anti-DNA Polymerase thet antibody A02205 on canine. You can run a BLAST between canine and the immunogen sequence of anti-DNA Polymerase thet antibody A02205 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine secondary oocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
Is this A02205 anti-DNA Polymerase thet antibody reactive to the isotypes of POLQ?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of A02205 anti-DNA Polymerase thet antibody is A peptide derived from human POLQ. Immunogen sequence location: 181-230. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
Does anti-DNA Polymerase thet antibody A02205 work for IHC with spleen?
A. Huang
Verified customer
Asked: 2019-03-25
Answer
According to the expression profile of spleen, POLQ is highly expressed in spleen. So, it is likely that anti-DNA Polymerase thet antibody A02205 will work for IHC with spleen.
Boster Scientific Support
Answered: 2019-03-25
Question
We are currently using anti-DNA Polymerase thet antibody A02205 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
The anti-DNA Polymerase thet antibody (A02205) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-04
Question
I was wanting to use your anti-DNA Polymerase thet antibody for IHC for human spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human spleen identification?
Verified Customer
Verified customer
Asked: 2017-07-14
Answer
You can see on the product datasheet, A02205 anti-DNA Polymerase thet antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-14
