Anti-DNA topoisomerase 1 TOP1 Antibody

Product Name

Anti-DNA topoisomerase 1 TOP1 Antibody

View all Topoisomerase I Antibodies

SKU/Catalog Number

A00434-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-DNA topoisomerase 1 TOP1 Antibody catalog # A00434-2. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DNA topoisomerase 1 TOP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00434-2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Recombinant Protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00434-2 is reactive to TOP1 in Human, Mouse

Applications

A00434-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

90.726kDa

Background of Topoisomerase I

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP)  is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules.  FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:5,000
ICC: 1:50-1:500
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of TOP1 using Anti-DNA topoisomerase 1 TOP1 Antibody (A00434-2).

ICC staining TOP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-DNA topoisomerase 1 TOP1 Antibody A00434-2

Western blot analysis of TOP1 on different lysates using anti-TOP1 antibody at 1/1

Gene/Protein Information For TOP1 (Source: Uniprot.org, NCBI)

Alternative Names

DNA topoisomerase 1; DNA topoisomerase I; EC 5.99.1.2; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase TOP1 TOPI DNA topoisomerase I DNA topoisomerase 1|Scl-70 antigen|topoisomerase (DNA) I|type I DNA topoisomerase

For more info on TOP1, check out the TOP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TOP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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