Anti-DNAJC5 Antibody Picoband™

Product Name

Anti-DNAJC5 Antibody Picoband™

View all CSP Antibodies

SKU/Catalog Number

A04220-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-DNAJC5 Antibody Picoband™ catalog # A04220-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-DNAJC5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04220-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human DNAJC5 recombinant protein (Position: A2-N198).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A04220-2 is reactive to Dnajc5 in Human, Mouse

Applications

A04220-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

22.101kDa

Background of CSP

DnaJ homolog subfamily C member 5, also known as cysteine string protein or CSP is a protein, that in humans encoded by the DNAJC5 gene. This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. The encoded protein plays a role in membrane trafficking and protein folding, and has been shown to have anti-neurodegenerative properties. The encoded protein is known to play a role in cystic fibrosis and Huntington's disease. A pseudogene of this gene is located on the short arm of chromosome 8.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of DNAJC5 using anti-DNAJC5 antibody (A04220-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC5 antigen affinity purified polyclonal antibody (Catalog # A04220-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC5 at approximately 24 kDa. The expected band size for DNAJC5 is at 22 kDa.

Gene/Protein Information For Dnajc5 (Source: Uniprot.org, NCBI)

Alternative Names

CSP; cysteine string protein alpha; Cysteine string protein; DKFZp434N1429; DKFZp761N1221; DnaJ (Hsp40) homolog, subfamily C, member 5; dnaJ homolog subfamily C member 5; DNAJC5A; FLJ00118; FLJ13070 Dnajc5|2610314I24Rik, AU018536, C, Csp|DnaJ heat shock protein family (Hsp40) member C5|dnaJ homolog subfamily C member 5|DnaJ (Hsp40) homolog, subfamily C, member 5|cysteine string protein

For more info on Dnajc5, check out the Dnajc5 Infographic

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In this infographic, you will see the following information for Dnajc5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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