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Product Info Summary
| SKU: | M01629-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-DNM2 Antibody (N-Term)
SKU/Catalog Number
M01629-1
Size
50 µl
Description
Boster Bio Anti-DNM2 Antibody (N-Term) (Catalog # M01629-1). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DNM2 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01629-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This DNM2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 213-247 amino acids from human DNM2.
Reactive Species
M01629-1 is reactive to DNM2 in Human, Mouse
Calculated molecular weight
98.1 kDa
Background of DNM2
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01629-1 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling DNM2 with M01629-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-DNM2 Antibody (N-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: mouse brain lysate
Lane 5: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 98 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with M01629-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M01629-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-DNM2 Antibody (N-Term) (M01629-1)
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