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- Table of Contents
DNMT1 antibody (M00172) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.
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Anti-Dnmt1 Rabbit Monoclonal Antibody
DNMT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
DNMT1 antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human HepG2 whole cell, human 293T whole cell, rat NRK whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
DNMT1 antibody was positively detected by IHC in:
Human glioblastoma tissue, Rat spleen tissue, Mouse lung tissue, Human lung large cell cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Dnmt1 Rabbit Monoclonal Antibody
View all DNMT1 Antibodies
View all DNMT1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AODB-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Dnmt1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
200 kDa
Calculated Molecular Weight
183.2 kDa
Cite This Product
Anti-Dnmt1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00172)
Protocols
Western Blot protocol for DNMT1 antibody
Immunohistochemistry (IHC) protocol for DNMT1 antibody
Publications
Application:
Species:
DNMT3A-dependent suppression of HIF-1α alleviates myopia by reducing scleral ferroptosis and fibroblast extracellular matrix remodeling
Published: 2025-12-15
Journal: AMERICAN JOURNAL OF PATHOLOGY
Application: WB,IHC
Impact Factor: 3.60
Species: Rat
PMID: 41407196
Supplementation of l-arginine in pregnant gilts affects the protein abundance of DNMT1 in 35-day fetuses
Published: 2024-08-08
Journal: ANIMAL REPRODUCTION SCIENCE
Application: WB
Impact Factor: 2.20
Species: Pig
PMID: 39167962
Melatonin prevents the binding of vascular endothelial growth factor to its receptor and promotes the expression of extracellular matrix‑associated genes in nucleus pulposus cells
Published: 2020-09-18
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 1.79
Species: Human
PMID: 32989385
β-Cypermethrin Alleviated the Inhibitory Effect of Medium from RAW 264.7 Cells on 3T3-L1 Cell Maturation into Adipocytes
Published: 2020-03-31
Journal: LIPIDS
Application: WB
Impact Factor: 1.92
Species: Mouse
PMID: 32236964
Review
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Product Promise
We promise Anti-Dnmt1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00172
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026