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Product Info Summary
| SKU: | M00172 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Dnmt1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00172
BM4917 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Dnmt1 Rabbit Monoclonal Antibody catalog # M00172. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dnmt1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00172)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AODB-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Dnmt1
Reactive Species
M00172 is reactive to DNMT1 in Human, Mouse, Rat
Observed Molecular Weight
200 kDa
Calculated molecular weight
183.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00172 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human HepG2 whole cell, human 293T whole cell, rat NRK whole cell, mouse NIH/3T3 whole cell
IHC: Human glioblastoma tissue, Rat spleen tissue, Mouse lung tissue, Human lung large cell cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of DNMT1 using anti-DNMT1 antibody (M00172).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT1 antigen affinity purified monoclonal antibody (M00172) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNMT1 at approximately 200 kDa. The expected band size for DNMT1 is at 183 kDa.
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Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat spleen, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:100 dilution.
Specific Publications For Anti-Dnmt1 Rabbit Monoclonal Antibody (M00172)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Dnmt1 Rabbit Monoclonal Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Dnmt1 Rabbit Monoclonal antibody M00172. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-09
Question
Is this M00172 anti-Dnmt1 Rabbit Monoclonal antibody reactive to the isotypes of DNMT1?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The immunogen of M00172 anti-Dnmt1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Dnmt1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-30
Question
Would anti-Dnmt1 Rabbit Monoclonal antibody M00172 work for IF with liver?
T. Rodriguez
Verified customer
Asked: 2019-10-07
Answer
According to the expression profile of liver, DNMT1 is highly expressed in liver. So, it is likely that anti-Dnmt1 Rabbit Monoclonal antibody M00172 will work for IF with liver.
Boster Scientific Support
Answered: 2019-10-07
Question
Is a blocking peptide available for product anti-Dnmt1 Rabbit Monoclonal antibody (M00172)?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We do provide the blocking peptide for product anti-Dnmt1 Rabbit Monoclonal antibody (M00172). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-30
Question
We ordered your anti-Dnmt1 Rabbit Monoclonal antibody for WB on cervix carcinoma erythroleukemia in a previous experiment. I am using rat, and We intend to use the antibody for IF next. I would like examining cervix carcinoma erythroleukemia as well as leukemic t-cell in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
I have checked the website and datasheets of our anti-Dnmt1 Rabbit Monoclonal antibody and it seems that M00172 has been validated on rat in both WB and IF. Thus M00172 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-24
Question
I was wanting to use your anti-Dnmt1 Rabbit Monoclonal antibody for IF for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
It shows on the product datasheet, M00172 anti-Dnmt1 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-17
Question
Do you have a BSA free version of anti-Dnmt1 Rabbit Monoclonal antibody M00172 available?
R. Huang
Verified customer
Asked: 2018-10-22
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Dnmt1 Rabbit Monoclonal antibody M00172 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-22
Question
Can you help my question with product M00172, anti-Dnmt1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Zhang
Verified customer
Asked: 2018-07-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00172 anti-Dnmt1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-10
Question
My team were satisfied with the WB result of your anti-Dnmt1 Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleus. using this antibody. Is that expected? Could you tell me where is DNMT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
From what I have seen in literature, cervix carcinoma does express DNMT1. Generally DNMT1 expresses in nucleus. Regarding which tissues have DNMT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostatic carcinoma, Pubmed ID: 15057824
Boster Scientific Support
Answered: 2018-01-09
Question
I am looking for to test anti-Dnmt1 Rabbit Monoclonal antibody M00172 on mouse liver for research purposes, then I may be interested in using anti-Dnmt1 Rabbit Monoclonal antibody M00172 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
The products we sell, including anti-Dnmt1 Rabbit Monoclonal antibody M00172, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-09
Question
I see that the anti-Dnmt1 Rabbit Monoclonal antibody M00172 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-02
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-08-02
Question
Does M00172 anti-Dnmt1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
You can see on the product datasheet, M00172 anti-Dnmt1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-28
Question
I have attached the WB image, lot number and protocol we used for liver using anti-Dnmt1 Rabbit Monoclonal antibody M00172. Please let me know if you require anything else.
L. Krishna
Verified customer
Asked: 2016-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-23
Question
We have seen staining in mouse prostatic carcinoma. What should we do? Is anti-Dnmt1 Rabbit Monoclonal antibody supposed to stain prostatic carcinoma positively?
L. Collins
Verified customer
Asked: 2015-09-15
Answer
From what I have seen in literature prostatic carcinoma does express DNMT1. From what I have seen in Uniprot.org, DNMT1 is expressed in oocyte, prostatic carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have DNMT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostatic carcinoma, Pubmed ID: 15057824
Boster Scientific Support
Answered: 2015-09-15
Question
We are currently using anti-Dnmt1 Rabbit Monoclonal antibody M00172 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
A. Collins
Verified customer
Asked: 2014-03-19
Answer
The anti-Dnmt1 Rabbit Monoclonal antibody (M00172) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-19
