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Product Info Summary
| SKU: | M00136 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Dnmt3a Rabbit Monoclonal Antibody
SKU/Catalog Number
M00136
BM4372 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Dnmt3a Rabbit Monoclonal Antibody catalog # M00136. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dnmt3a Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00136)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EOI-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Dnmt3a
Reactive Species
M00136 is reactive to DNMT3A in Human, Mouse, Rat
Observed Molecular Weight
130 kDa
Calculated molecular weight
101.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00136 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of Dnmt3a using anti-Dnmt3a antibody (M00136).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dnmt3a antigen affinity purified monoclonal antibody (Catalog # M00136) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dnmt3a at approximately 130 kDa. The expected band size for Dnmt3a is at 130 kDa.
Specific Publications For Anti-Dnmt3a Rabbit Monoclonal Antibody (M00136)
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3 Customer Q&As for Anti-Dnmt3a Rabbit Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for fetal testis using anti-Dnmt3a Rabbit Monoclonal antibody M00136. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-30
Question
Will anti-Dnmt3a Rabbit Monoclonal antibody M00136 work for WB with fetal testis?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
According to the expression profile of fetal testis, DNMT3A is highly expressed in fetal testis. So, it is likely that anti-Dnmt3a Rabbit Monoclonal antibody M00136 will work for WB with fetal testis.
Boster Scientific Support
Answered: 2019-01-08
Question
I see that the anti-Dnmt3a Rabbit Monoclonal antibody M00136 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-05-23
