Product Info Summary
SKU: | A03039-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Dok-1 Antibody
SKU/Catalog Number
A03039-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Dok-1 Antibody catalog # A03039-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dok-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03039-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Dok-1 around the non-phosphorylation site of Y362.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03039-1 is reactive to DOK1 in Human, Mouse, Rat
Applications
A03039-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
52.392kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of Jurkat cells using Dok-1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For DOK1 (Source: Uniprot.org, NCBI)
Gene Name
DOK1
Full Name
Docking protein 1
Weight
52.392kDa
Superfamily
DOK family
Alternative Names
docking protein 1 (downstream of tyrosine kinase 1); docking protein 1; docking protein 1, 62kD (downstream of tyrosine kinase 1); docking protein 1, 62kDa (downstream of tyrosine kinase 1); Downstream of tyrosine kinase 1; MGC117395; MGC138860; p62(dok); P62DOK; pp62 DOK1 P62DOK, pp62 docking protein 1 docking protein 1|Downstream of tyrosine kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DOK1, check out the DOK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DOK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dok-1 Antibody (A03039-1)
Hello CJ!
A03039-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Talin promotes integrin activation accompanied by generation of tension in talin and an increase in osmotic pressure in neurite outgrowth
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Dok-1 Antibody
Question
My question regarding product A03039-1, anti-Dok-1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03039-1 anti-Dok-1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-17
Question
I see that the anti-Dok-1 antibody A03039-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-03
Question
We are currently using anti-Dok-1 antibody A03039-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
The anti-Dok-1 antibody (A03039-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-19
Question
Is there a BSA free version of anti-Dok-1 antibody A03039-1 available?
A. Anderson
Verified customer
Asked: 2017-10-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Dok-1 antibody A03039-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-19
Question
Would A03039-1 anti-Dok-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
You can see on the product datasheet, A03039-1 anti-Dok-1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-20
Question
I have attached the WB image, lot number and protocol we used for tendon using anti-Dok-1 antibody A03039-1. Please let me know if you require anything else.
M. Li
Verified customer
Asked: 2015-01-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-14