Anti-DPAGT1 Antibody

Product Name

Anti-DPAGT1 Antibody

View all DPAGT1 Antibodies

SKU/Catalog Number

A08142

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DPAGT1 Antibody (Catalog # A08142). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

DPAGT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DPAGT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08142)

Host

Rabbit

Contents

DPAGT1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DPAGT1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human DPAGT1. The immunogen is located within amino acids 30 - 80 of DPAGT1.

Cross-reactivity

At least four isoforms of DPAGT1 are known to exist; this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.

Reactive Species

A08142 is reactive to DPAGT1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

46.1 kDa

Background of DPAGT1

The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08142 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DPAGT1 antibody can be used for detection of DPAGT1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information DPAGT1

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