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Product Info Summary
| SKU: | A10283-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-DPPA4 Antibody (N-term)
SKU/Catalog Number
A10283-2
Size
80 µl
Description
Boster Bio Anti-DPPA4 Antibody (N-term) (Catalog # A10283-2). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DPPA4 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10283-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This DPPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the N-terminal region of human DPPA4.
Reactive Species
A10283-2 is reactive to DPPA4 in Human
Calculated molecular weight
33.5 kDa
Background of DPPA4
DppA4 is a member of the Dppa family of developmental pluripotency associated transcription factors. Dppa4 is a target of Oct4, Sox2 and Nanog. It may play a role in maintaining cell pluripotentiality.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10283-2 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Anti-DPPA4 Antibody (N-term) at 1:2000 dilution + NCCIT whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 34 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of DPPA4 Antibody (N-term) in CEM cell line lysates (35ug/lane). DPPA4 (arrow) was detected using the purified Pab.
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A10283-2 staining DPPA4 in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-DPPA4 Antibody (N-term) (A10283-2)
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