Anti-Drosha Rabbit Monoclonal Antibody

Drosha antibody

Boster Bio Anti-Drosha Rabbit Monoclonal Antibody catalog # M00111. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00111
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Drosha Rabbit Monoclonal Antibody

View all Drosha Antibodies

SKU/Catalog Number

M00111

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Drosha Rabbit Monoclonal Antibody catalog # M00111. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Drosha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00111)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABEF-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Drosha

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00111 is reactive to Drosha in Human

Applications

M00111 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

63 kDa

Calculated molecular weight

158.828kDa

Background of Drosha

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For Drosha (Source: Uniprot.org, NCBI)

Gene Name

Drosha

Full Name

Ribonuclease 3

Weight

158.828kDa

Superfamily

ribonuclease III family

Alternative Names

drosha, double-stranded RNA-specific endoribonuclease; drosha, ribonuclease type III; EC 3.1.26.3; ETOHI2; nuclear RNase III Drosha; p241; Protein Drosha; putative protein p241 which interacts with transcription factor Sp1; putative ribonuclease III; RANSE3L; ribonuclease 3; Ribonuclease III; ribonuclease III, nuclear; RN3nuclear; RNase III Drosha|1110013A17Rik, AI874853, Etoh, Etohi2, R, Rn3, Rnasen|drosha, ribonuclease type III|ribonuclease 3|RNase III|ethanol induced 2|protein Drosha|ribonuclease III, nuclear

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Drosha, check out the Drosha Infographic

Drosha infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Drosha: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Drosha Rabbit Monoclonal Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-Drosha Rabbit Monoclonal antibody M00111. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-20

Question

We are currently using anti-Drosha Rabbit Monoclonal antibody M00111 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-15

Answer

The anti-Drosha Rabbit Monoclonal antibody (M00111) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-15

Question

See below the WB image, lot number and protocol we used for cerebellum using anti-Drosha Rabbit Monoclonal antibody M00111. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-24

Order DetailsPrice
M00111

100μL

$370
M00111-30ul

30μL sample

$99
M00111-carrier-free

Carrier Free

$370

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