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Product Info Summary
| SKU: | PA1762 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DUSP3 Antibody Picoband®
SKU/Catalog Number
PA1762
BA3748-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DUSP3 Antibody catalog # PA1762. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DUSP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1762)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DUSP3, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1762 is reactive to DUSP3 in Human, Mouse, Rat
Observed Molecular Weight
20 kDa
Calculated molecular weight
20.5 kDa
Background of DUSP3
DUSP3 (Dual-specificity phosphatase 3), also called VHR, is a member of the dual specificity protein phosphatase subfamily. DUSPs constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. DUSP3 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs. The DUSP3 gene is mapped on 17q21.31. Confocal microscopy demonstrated that phosphorylated VHR accumulated at the immune synapse between the T cell and the antigen-presenting cell in the presence of antigen. Tyrosine phosphorylation of VHR affects protein-protein interaction, subcellular location, or substrate targeting, given that tyr138 is located on the opposite side of the VHR catalytic center.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1762 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human MCF-7 whole cell, human MDA-MB-453 whole cell, human Caco-2 whole cell, human HepG2 whole cell, rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue
Validation Images & Assay Conditions
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Western blot analysis of DUSP3 using anti-DUSP3 antibody (PA1762).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human MDA-MB-453 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP3 antigen affinity purified polyclonal antibody (Catalog # PA1762) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DUSP3 at approximately 20 kDa. The expected band size for DUSP3 is at 20 kDa.
Specific Publications For Anti-DUSP3 Antibody Picoband® (PA1762)
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4 Customer Q&As for Anti-DUSP3 Antibody Picoband®
Question
Would PA1762 anti-DUSP3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Gonzalez
Verified customer
Asked: 2019-12-20
Answer
It shows on the product datasheet, PA1762 anti-DUSP3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-DUSP3 antibody PA1762 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
E. Banerjee
Verified customer
Asked: 2019-09-10
Answer
The anti-DUSP3 antibody (PA1762) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-10
Question
Will anti-DUSP3 antibody PA1762 work on canine WB with duodenum uterus?
J. Huang
Verified customer
Asked: 2015-03-24
Answer
Our lab technicians have not validated anti-DUSP3 antibody PA1762 on canine. You can run a BLAST between canine and the immunogen sequence of anti-DUSP3 antibody PA1762 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine duodenum uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-24
Question
Is a blocking peptide available for product anti-DUSP3 antibody (PA1762)?
P. Singh
Verified customer
Asked: 2013-05-24
Answer
We do provide the blocking peptide for product anti-DUSP3 antibody (PA1762). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-05-24
