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Product Info Summary
| SKU: | M03059 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-DYNC1H1 Antibody (C-term)
SKU/Catalog Number
M03059
Size
80 µl
Description
Boster Bio Anti-DYNC1H1 Antibody (C-term) (Catalog # M03059). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-DYNC1H1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03059)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This DYNC1H1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4202-4236 amino acids from the C-terminal region of human DYNC1H1.
Reactive Species
M03059 is reactive to DYNC1H1 in Human, Mouse
Calculated molecular weight
532.4 kDa
Background of DYNC1H1
Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03059 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution
Lane 1: U-87 MG whole cell lysates
Lane 2: PC-3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 532 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 532 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M03059 staining DYNC1H1 in human testis sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-DYNC1H1 Antibody (C-term) (M03059)
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